An intravital and confocal microscopic study of the distribution of intracameral antigen in the aqueous outflow pathways and limbus of the rat eye

Experimental Eye Research
Serge CameloPaul G McMenamin

Abstract

In a previous investigation into the fate of fluorescently labelled antigen (Ag) injected into the anterior chamber (AC) of the rat eye, a large number of Ag+ cells were noted in the conventional and non-conventional aqueous humour outflow pathways together with the external limbus. The aim of this study was to investigate the precise distribution and phenotype of these cells and compare their ability to capture fluorescent-labelled protein (bovine serum albumin, BSA, and ovalbumin, OVA) and polysaccharides (dextran, Dx) injected into the AC. The density of Ag+ cells in the iris and limbus was investigated using in vivo video fluorescence microscopy 24 hr post-injection. The distribution and phenotype of Ag+ cells in ocular tissues was analysed by confocal microscopy of frozen sections and in iris and corneoscleral/limbal wholemounts from animals sacrificed 24 hr post injection. The general distribution of labelled Ag was equivalent in OVA, BSA and Dx injected animals. Antigen-bearing cells were observed within the iris, iridocorneal angle, pre-equatorial choroid and around limbal/episcleral vessels. Localization of Ag+ cells and free Ag in the anterior segment suggests that substances of these molecular weights (40-70 kDa) lea...Continue Reading

References

Oct 11, 1974·Nature·H J Kaplan, J W Streilein
Jun 1, 1972·American Journal of Ophthalmology·H InomataG K Smelser
Sep 14, 1994·Journal of Immunological Methods·C D DijkstraJ G Damoiseaux
Apr 27, 1999·The British Journal of Ophthalmology·P G McMenamin
Jan 16, 2002·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·F HoffmannS E Coupland
Apr 26, 2003·Investigative Ophthalmology & Visual Science·Matthias D BeckerJames T Rosenbaum
Nov 26, 2003·Investigative Ophthalmology & Visual Science·Serge CameloPaul G McMenamin

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Citations

Feb 21, 2013·Investigative Ophthalmology & Visual Science·Swarup S SwaminathanDouglas J Rhee
Jul 22, 2008·Experimental Eye Research·Kevin C ChanEd X Wu
Nov 6, 2008·Clinical & Experimental Ophthalmology·Paul G McMenamin
Mar 3, 2010·Immunological Reviews·John V ForresterPaul G McMenamin
Aug 29, 2006·Experimental Eye Research·Paul G McMenaminSerge Camelo
Jun 2, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Laure LajavardiAmélie Bochot
Jan 1, 2016·Vestnik oftalmologii·S V TrufanovD A Krakhmaleva
Dec 1, 2020·Progress in Retinal and Eye Research·Ester Reina-TorresW Daniel Stamer

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