PMID: 11334965May 4, 2001Paper

An investigation into the suitability of silica beads for cell separations based on density perturbation

Journal of Immunological Methods
L Bildirici, D Rickwood

Abstract

This study has investigated possible alternative types of beads for fractionating cells on the basis of density perturbation. It is well known that uniform magnetic beads can be extremely important tools for separating cells by both magnetic separation techniques and density perturbation. However, because of the inherent expense associated with the use of magnetic beads, it was decided to study the possible use of inexpensive silica beads for density perturbation in terms of their attachment and modification of density of cells and to compare them with uniform Dynabeads. Silica beads were analyzed to determine their size and effect on the density of cells. Differentiated HL60 cells were used as a model system. As differentiation occurs, different levels of antigens are expressed on the cell surface and this results in different numbers of beads binding to cells. DMSO-differentiated HL60 cells were mixed with anti-CD11b-coated beads at a ratio of 20:1 (beads/cell), and gentle mixing was carried out at 20 degrees C on the end-over-end mixer. The binding of antibody-coated silica beads and Dynabeads to partially differentiated HL60 cells were compared. The conclusions reached on the basis of these experiments are that antibody-coa...Continue Reading

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S J CollinsR C Gallo
Aug 9, 1993·Journal of Immunological Methods·D PatelD Rickwood
Sep 15, 1995·Clinica Chimica Acta; International Journal of Clinical Chemistry·D PatelD Rickwood
Jun 16, 2000·Journal of Immunological Methods·L Bildirici, D Rickwood
Feb 1, 1984·Immunology Today·M Wilchek, E A Bayer

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