Abstract
Salp15, a 15-kDa tick salivary gland protein, has several suppressive modes of activity against host immunity and plays a critical role in the transmission of Lyme disease spirochetes in Ixodes scapularis and Ixodes ricinus, major vectors of Lyme disease in North America and Western Europe. Salp15 adheres to Borrelia burgdorferi and specifically interacts with its outer surface protein C (OspC), protecting the spirochete from antibody-mediated cytotoxicity and facilitating infection in the mice. Recently, we identified two Salp15 homologues, IperSalp15-1 and IperSalp15-2, in Ixodes persulcatus, a vector for Lyme disease in Japan. Here we describe the function of IperSalp15 in the transmission of Lyme borreliosis. To investigate the function of IperSalp15, recombinant IperSalp15-1 and IperSalp15-2 were prepared in bacterial and insect cells. Both were identified in the sera of tick-immunized hamsters, indicating that these are secretory proteins in exposed host animals. Solid-phase overlay and indirect fluorescence assays showed that IperSalp15 binds to OspC from B. burgdorferi, Borrelia garinii, and Borrelia afzelii. Importantly, this binding likely protected the spirochete from antibody-mediated cytotoxicity in vitro. In addit...Continue Reading
References
May 1, 1994·Journal of Medical Entomology·M Nakao, K Miyamoto
Mar 7, 2001·The EMBO Journal·D KumaranS Swaminathan
Sep 28, 2001·The Journal of Infectious Diseases·S DasE Fikrig
Jul 18, 2002·Immunity·Juan AnguitaErol Fikrig
Nov 25, 2003·Lancet·Gerold Stanek, Franc Strle
Apr 16, 2004·The Journal of Clinical Investigation·Allen C SteereLisa Glickstein
Jul 29, 2005·Nature·Nandhini RamamoorthiErol Fikrig
Jul 18, 2006·Parasite Immunology·J de la Fuente, K M Kocan
Oct 10, 2006·Clinical and Vaccine Immunology : CVI·Eric L BucklesRichard T Marconi
Mar 9, 2007·FEMS Immunology and Medical Microbiology·Ignacio J JuncadellaJuan Anguita
Jul 28, 2007·Trends in Parasitology·Joppe W R HoviusErol Fikrig
Oct 10, 2007·Veterinary Research Communications·J de la FuenteE F Blouin
Sep 28, 2007·Vector Borne and Zoonotic Diseases·J W R HoviusE Fikrig
Dec 29, 2007·Biochemical and Biophysical Research Communications·Ignacio J JuncadellaJuan Anguita
Feb 15, 2008·PLoS Medicine·Joppe W R HoviusErol Fikrig
Feb 20, 2008·PLoS Pathogens·Joppe W R HoviusTeunis B H Geijtenbeek
Apr 23, 2008·Infection and Immunity·Tim J SchuijtAlje P van Dam
Nov 18, 2009·Cell Host & Microbe·Jianfeng DaiErol Fikrig
Dec 8, 2009·Journal of Medical Entomology·Andrias HojgaardJoseph Piesman
Mar 6, 2010·Insect Molecular Biology·A MoriK Ohashi
Mar 27, 2012·Ticks and Tick-borne Diseases·Satoru KonnaiKazuhiko Ohashi
Oct 11, 2012·The Journal of Veterinary Medical Science·Yusuke MuraseKazuhiko Ohashi
Feb 28, 2014·Ticks and Tick-borne Diseases·J LiuM A Bakheit
Apr 5, 2014·Insect Molecular Biology·A HidanoK Ohahsi
Apr 10, 2014·PloS One·Xin WangWu-Chun Cao
Aug 12, 2014·PloS One·Ai TakanoHiroki Kawabata
Citations
Jul 12, 2017·Frontiers in Cellular and Infection Microbiology·Ladislav ŠimoSarah I Bonnet
Jan 31, 2020·Frontiers in Immunology·Shiyuan WenFukai Bao
Feb 6, 2017·Frontiers in Immunology·Brandee L Stone, Catherine A Brissette
Jan 6, 2021·Ticks and Tick-borne Diseases·Paulina BierwagenAnna Urbanowicz