An investigation of binding ability of Ixodes persulcatus Schulze Salp15 with Lyme disease spirochetes

Insect Biochemistry and Molecular Biology
Y MuraseKazuhko Ohashi

Abstract

Salp15, a 15-kDa tick salivary gland protein, has several suppressive modes of activity against host immunity and plays a critical role in the transmission of Lyme disease spirochetes in Ixodes scapularis and Ixodes ricinus, major vectors of Lyme disease in North America and Western Europe. Salp15 adheres to Borrelia burgdorferi and specifically interacts with its outer surface protein C (OspC), protecting the spirochete from antibody-mediated cytotoxicity and facilitating infection in the mice. Recently, we identified two Salp15 homologues, IperSalp15-1 and IperSalp15-2, in Ixodes persulcatus, a vector for Lyme disease in Japan. Here we describe the function of IperSalp15 in the transmission of Lyme borreliosis. To investigate the function of IperSalp15, recombinant IperSalp15-1 and IperSalp15-2 were prepared in bacterial and insect cells. Both were identified in the sera of tick-immunized hamsters, indicating that these are secretory proteins in exposed host animals. Solid-phase overlay and indirect fluorescence assays showed that IperSalp15 binds to OspC from B. burgdorferi, Borrelia garinii, and Borrelia afzelii. Importantly, this binding likely protected the spirochete from antibody-mediated cytotoxicity in vitro. In addit...Continue Reading

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Citations

Jul 12, 2017·Frontiers in Cellular and Infection Microbiology·Ladislav ŠimoSarah I Bonnet
Jan 31, 2020·Frontiers in Immunology·Shiyuan WenFukai Bao
Feb 6, 2017·Frontiers in Immunology·Brandee L Stone, Catherine A Brissette

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