An investigation of liquid carryover and sample residual for a high-throughput flow cytometer sample delivery system

Analytical Chemistry
Joseph W BartschBruce S Edwards

Abstract

The phenomenon of intersample contamination in air-segmented continuous-flow assays has been studied for many years, and new uses are being found for these sampling techniques every day. One application that has been developed recently employs a flow cytometer to conduct high-throughput screening assays of biological compounds. We have explored the sources of intersample contamination in the system and shown how methods developed previously can be applied to describe these phenomena. Using a simple model, we were able to accurately measure liquid film thickness in the sample tubing and demonstrate the effects of intersample contamination in a flow cytometer assay. Also, measures have been taken to reduce the level of intersample contamination in cytometric screening assays, helping to make the system a more viable tool for drug screening applications.

References

May 29, 2003·Combinatorial Chemistry & High Throughput Screening·A WallerL A Sklar
Sep 1, 1957·American Journal of Clinical Pathology·L T SKEGGS
Mar 10, 2004·Journal of Biomolecular Screening·Susan M YoungBruce S Edwards

❮ Previous
Next ❯

Citations

Aug 21, 2012·Nature Biotechnology·Bernd BodenmillerGarry P Nolan
Sep 2, 2010·Assay and Drug Development Technologies·Larry Sklar, Bruce Edwards
May 1, 2007·Expert Opinion on Drug Discovery·Bruce S EdwardsLarry A Sklar
Mar 23, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Bruce S EdwardsLarry A Sklar
Aug 4, 2004·Current Opinion in Chemical Biology·Bruce S EdwardsLarry A Sklar
Mar 26, 2015·Journal of Biomolecular Screening·Bruce S Edwards, Larry A Sklar
May 7, 2010·Lab on a Chip·Jenifer Clausell-TormosChristoph A Merten
Jun 21, 2005·Journal of Biomolecular Screening·Susan M YoungBruce S Edwards
Jan 31, 2008·Analytical Chemistry·Michiel T KreutzerKlavs F Jensen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Sergio RamirezBruce S Edwards
© 2022 Meta ULC. All rights reserved