PMID: 11342250May 9, 2001Paper

An investigation of the action of porcine pancreatic alpha-amylase on native and gelatinised starches

Biochimica Et Biophysica Acta
Suzanne L SlaughterPeter J Butterworth

Abstract

The action of pancreatic alpha-amylase (EC 3.2.1.1) on various starches has been studied in order to achieve better understanding of how starch structural properties influence enzyme kinetic parameters. Such studies are important in seeking explanations for the wide differences reported in postprandial glycaemic and insulinaemic indices associated with different starchy foodstuffs. Using starches from a number of different sources, in both native and gelatinised forms, as substrates for porcine alpha-amylase, we showed by enzyme kinetic studies that adsorption of amylase to starch is of kinetic importance in the reaction mechanism, so that the relationship between reaction velocity and enzyme concentration [E0] is logarithmic and described by the Freundlich equation. Estimations of catalytic efficiencies were derived from measurements of kcat/Km performed with constant enzyme concentration so that comparisons between different starches were not complicated by the logarithmic relationship between E0 and reaction velocity. Such studies reveal that native starches from normal and waxy rice are slightly better substrates than those from wheat and potato. After gelatinisation at 100 degrees C, kcat/Km values increased by 13-fold (wa...Continue Reading

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Citations

Jul 7, 2011·International Journal of Molecular Sciences·Maria R Romero-LopezAurea Bernardino-Nicanor
Apr 29, 2014·Expert Review of Vaccines·Sergio Rosales-Mendoza, Jorge A Salazar-González
Dec 22, 2015·European Journal of Nutrition·M R Jaime-FonsecaS Bakalis
May 9, 2009·Journal of Ethnopharmacology·M S DeutschländerN Lall
Oct 17, 2015·Food Chemistry·Erich von Borries-MedranoMiguel A Aguilar-Méndez
Mar 10, 2015·Critical Reviews in Food Science and Nutrition·Sushil DhitalMichael J Gidley
Oct 3, 2003·International Journal of Food Sciences and Nutrition·Naofumi KitabatakeYasuyuki Oi
Jul 16, 2011·Food & Function·Tara KinnearBenoit Bizimungu
Feb 8, 2020·Journal of the Science of Food and Agriculture·Rizliya VisvanathanRamiah Sivakanesan
Aug 24, 2004·The American Journal of Clinical Nutrition·Peter R EllisDavid J A Jenkins
Sep 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Giang T Nguyen, Peter A Sopade
Jan 5, 2021·Annual Review of Food Science and Technology·Ming Miao, Bruce R Hamaker
Mar 31, 2015·Biomacromolecules·Andrew J BaldwinPeter R Ellis
Jul 17, 2019·Journal of Agricultural and Food Chemistry·Yanhua WangLes Copeland

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