An investigation of the antigenic structure of Toxoplasma gondii

Parasite Immunology
H P Hughes, A H Balfour

Abstract

Mechanical fragmentation has been the prime method for preparing soluble Toxoplasma gondii antigens for use in serodiagnosis. The three methods most commonly used (lysis-in-water, sonication and freeze-thawing) all yielded three antigenic peaks (4, 5 and 6) on crossed immunoelectrophoresis. Antigen 6 has been shown to be heat stable (56 degrees C/1 h) and appears to be a major antigen in the indirect haemagglutination test (IHAT). Saponin and octyl glucoside were found to be the most effective detergents for solubilization when used in combination, yielding between 7 and 11 antigens. Using a new triple-staining technique, antigen 3 has been characterized as a glycoprotein, antigen 7 as a lipopolysacchiaride and antigens 1, 2, 4, 5, 6 and 8 to 11 as proteins. Molecular weight determination by gel filtration has been carried out and the majority of the antigens have a mol. wt of 10(5) to 1.5 x 10(5) daltons. Crossed immunoelectrofocusing has shown that all the antigens are isoelectric at acidic pH. The findings of this preliminary investigation into the basic antigenic structure of T. gondii are discussed in relation to future work and the rational development of serodiagnostic tests.

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Citations

Nov 1, 1984·Tropical Animal Health and Production·V ShkapC Greenblatt
Oct 1, 1982·International Journal for Parasitology·H P Hughes, F van Knapen
Aug 1, 1986·International Journal for Parasitology·H P HughesD G Fleck
Feb 1, 1987·International Journal for Parasitology·B ChumpitaziJ M Autheman
Mar 1, 1987·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·A HasslH Aspöck
Apr 1, 1990·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·A Hassl, H Aspöck
Nov 1, 1988·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·K Hermentin, H Aspöck
Jun 1, 1987·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·K LövgrenB Morein
May 1, 1997·Revista Do Instituto De Medicina Tropical De São Paulo·Y I YamamotoS Hoshino-Shimizi
Jun 1, 1997·European Journal of Immunology·H G FischerU Hadding
Dec 1, 1984·Zentralblatt für Veterinärmedizin. Reihe A·M M SuterP Oettli

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