PMID: 9546115Apr 18, 1998Paper

An investigation of the molecular basis of the spontaneous occurrence of a catalase-negative phenotype in Helicobacter pylori

Helicobacter
J ManosS L Hazell

Abstract

The discovery of a highly active catalase in Helicobacter pylori that in some strains may lose its activity has generated strong scientific interest. We have characterized a spontaneous catalase-negative isolate of H. pylori (UNSW-RU1) and sequenced katA in the parent strain and the promoters of both phenotypes as a prelude to understanding the genetic processes leading to the failure to express catalase. Protein extracts from both phenotypes were examined for catalase on 2D-PAGE and analyzed by Western blot-based immuno-analysis. Presence of catalase mRNA was detected by Northern blot. Hi-Fidelity PCR was used to sequence the katA promoter while katA was sequenced using cycle-sequencing. The transcription start site was located by primer extension. Catalase protein was absent in UNSW-RU1 (KatA-) by 2D-PAGE and Western blot, as was catalase mRNA by Northern blot, indicating that the cause of the KatA- phenotype was at the level of transcription. No mutations were found in the promoter region of the KatA- isolate. The transcription start site was identified 55 bp upstream of the ATG site and putative RNA polymerase binding sites were mapped at "-10" and "-35". A Fur box was identified 181 bp upstream of the transcription start s...Continue Reading

References

Sep 1, 1992·Journal of Gastroenterology and Hepatology·S NaliniK A Balasubramanian
Jun 1, 1992·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·T U WestblomB R Midkiff
Mar 1, 1992·The Journal of Nutrition·G Storz, L A Tartaglia
Jan 1, 1991·Journal of General Microbiology·S L HazellD Y Graham
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S M Naik, H M Hassan
May 25, 1990·Nucleic Acids Research·M E SchmittB L Trumpower
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M F ChristmanB N Ames
Dec 10, 1988·Gene·J G Belasco, C F Higgins
Jun 11, 1986·Nucleic Acids Research·Y Takeda, H Avila
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·I Fita, M G Rossmann
Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Sep 27, 1995·Biochimica Et Biophysica Acta·D J BicoutH M Jouve
Jan 1, 1993·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·L P AndersenH Nielsen
Jan 1, 1996·Nucleic Acids Research·A Bairoch, R Apweiler
Jan 1, 1996·Scandinavian Journal of Gastroenterology. Supplement·J E Crabtree
Nov 14, 1997·Infection and Immunity·F J RadcliffA Lee

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Citations

Jun 4, 2010·Antioxidants & Redox Signaling·Hitoshi TsugawaToshifumi Hibi
Aug 2, 2006·Molecular Microbiology·Ge WangRobert J Maier
Jul 3, 2002·Clinical and Diagnostic Laboratory Immunology·Nobuyuki SuzukiTakashi Shimoyama

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