An investigation on the cytotoxicity and caspase-mediated apoptotic effect of biologically synthesized gold nanoparticles using Cardiospermum halicacabum on AGS gastric carcinoma cells

International Journal of Nanomedicine
Chunfeng LiYingwei Xue

Abstract

Gastric cancer is the fourth most common cancer and second leading cause of cancer death worldwide. Cardiospermum halicacabum is used to treat nerve disorders, stiffness, rheumatism, ear ache, snake bite, and so on. In this study, the reaction parameters were optimized to control the size of the nanoparticle, which was confirmed by transmission electron microscopy. Various characterization techniques such as selected area diffraction pattern, UV-visible spectroscopy, energy-dispersive X-ray analysis, dynamic light scattering, Fourier-transform infrared spectroscopy, and atomic force microscopy were employed to analyze the synthesized AuNPs obtained from C. halicacabum (CH-AuNP) against gastric carcinoma cell line. The cytotoxic effect of CH-AuNP against AGS, SNU-5, and SNU-16 cell lines was detected by MTT assay. The induction of apoptosis by CH-AuNP in AGS was analyzed by double staining technique using TUNEL and DAPI staining assays. Further to confirm the molecular mechanism exhibited by CH-AuNP to induce apoptosis, the intracellular ROS level was assessed and immunoblotting was performed to assess the apoptotic signaling molecules that often deregulated in cancerous condition. The results clearly prove that CH-AuNP increase...Continue Reading

Citations

Nov 11, 2020·International Journal of Molecular Sciences·Sylwia NakoniecznaWirginia Kukula-Koch
Jan 20, 2020·Seminars in Cancer Biology·Ganji Purnachandra NagarajuMohammad Amjad Kamal
Feb 8, 2021·Journal of Cancer Research and Clinical Oncology·Lanxin JiangRunwei Yan
Dec 17, 2020·Frontiers in Molecular Biosciences·Gennaro SanitàAnnalisa Lamberti
Mar 20, 2021·Nanomedicine·Firdos Alam KhanDana Almohazey

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Methods Mentioned

BETA
electrophoresis
surface plasmon resonance
transmission electron microscopy
AFM
flow cytometry
surface resonance

Software Mentioned

CELLQuest
Graph Pad Prism
WINFIRST
Globocan

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