An inward rectifier K+ current modulates in neuroblastoma cells the tyrosine phosphorylation of the pp125FAK and associated proteins: role in neuritogenesis

Biochemical and Biophysical Research Communications
L BianchiM Olivotto

Abstract

The relationships between the integrin-mediated activation of inward rectifyier K+ channels (KIR), the phosphorylation of pp125FAK and the rescue of neuritogenesis were studied in 41A3 mouse neuroblastoma cells. Neuritogenesis, elicited by adhesion to FN-enriched substrata, was reversibly impaired by pretreating these cells with the tyrosine kinase inhibitor Herbimycin A. This impairment mimicked that operated by Cs+ ions, which selectively inhibited the integrin-mediated activation of KIR channels. Various phosphotyrosine containing cellular proteins underwent a marked increase upon cell adhesion to FN-coated dishes. This increase was significantly reduced by Cs+ addition. Immunoprecipitation of pp125FAK revealed that the phosphorylation of this kinase and several associated proteins was significantly and reversibly inhibited by Cs+, indicating that integrin-mediated activation of KIR channels is a limiting step upstream to the phosphorylation of pp125FAK in the commitment to neuritogenesis.

Citations

May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jeff H HogerAnne Hoger
Jun 1, 1996·Current Opinion in Neurobiology·E A Jonas, L K Kaczmarek
Jul 11, 2008·Journal of Cellular Physiology·Jian-Feng WeiShan Ping Yu
Dec 18, 2014·Biochimica Et Biophysica Acta·Nuria ComesAntonio Felipe
Nov 9, 2014·Cell and Tissue Research·Akikazu FujitaYoshihisa Kurachi
Dec 18, 2002·Annals of the New York Academy of Sciences·A CherubiniA Arcangeli
Jun 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M OlivottoE Wanke
Aug 15, 2002·American Journal of Physiology. Cell Physiology·Margaret Colden-Stanfield
Oct 21, 2004·Biochemical Society Transactions·A ArcangeliE Wanke
Jul 27, 2000·American Journal of Physiology. Cell Physiology·M Colden-Stanfield, M Scanlon
Aug 5, 1998·The American Journal of Physiology·M Colden-Stanfield, E K Gallin
Nov 15, 1996·Journal of Neuroscience Research·G R StevensW L Klein

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.