An inward-rectifying K+ current in clonal rat pituitary cells and its modulation by thyrotrophin-releasing hormone

The Journal of Physiology
C K BauerJ R Schwarz

Abstract

1. Voltage-dependent K+ currents were recorded in cultured tumour-derived anterior pituitary cells of the rat (GH3 cells) with the patch clamp technique. An inward-rectifying current is described which is found to be carried by K+. 2. In isotonic KCl, whole-cell inward K+ currents were elicited by hyperpolarizing pulses from a holding potential of -40 mV. These inward K+ currents showed time- and voltage-dependent inactivation at potentials more negative than -60 mV. Inactivation was faster and more complete at larger hyperpolarizations. Recovery from inactivation was also time- and voltage-dependent. It was faster and more complete with more positive potentials. Time course of inactivation and of recovery from inactivation could be fitted by single exponentials. 3. Two results showed that a steady inward K+ current is present at -40 mV. The holding current at -40 mV was reduced following complete inactivation of the inward K+ current during strong hyperpolarizing pulses, and the amplitude of maximum inward K+ current elicited by hyperpolarization increased after depolarizing pre-pulses of 5 s. The resting conductance was estimated to be 20-30% of the maximum inward-rectifying conductance. 4. The inward K+ current was drastical...Continue Reading

Citations

Jun 25, 1999·The Journal of Physiology·R SchäferJ R Schwarz
Feb 12, 2005·The Journal of Physiology·Patrick SturmChristiane K Bauer
Dec 13, 2005·The Journal of Physiology·Niklas M KirchbergerChristiane K Bauer
Nov 15, 2011·Pflügers Archiv : European journal of physiology·Günter GlassmeierJürgen R Schwarz
Apr 1, 1995·Pflügers Archiv : European journal of physiology·E WischmeyerA Karschin
Dec 13, 1996·The Journal of Biological Chemistry·N A CohenC G Nichols
Apr 2, 1999·The European Journal of Neuroscience·R SchönherrE Wanke
Jun 17, 1998·The Journal of General Physiology·P S PennefatherT E DeCoursey
Oct 4, 2000·Physiological Reviews·M E FreemanG Nagy
Jan 29, 2005·The Journal of Physiology·Wiebke HirdesJürgen R Schwarz
Aug 19, 2009·Pflügers Archiv : European journal of physiology·Wiebke HirdesChristiane K Bauer
Nov 11, 2008·Pflügers Archiv : European journal of physiology·Andreas FeigenspanReto Weiler
Mar 15, 2012·Pflügers Archiv : European journal of physiology·Luis CarreteroPedro Domínguez
Jan 16, 2018·The Journal of Physiology·Christiane K Bauer, Jürgen R Schwarz
Jun 1, 1996·The European Journal of Neuroscience·M Atzori, A Nistri
Mar 1, 1997·The American Journal of Physiology·S M Simasko, S Sankaranarayanan
May 17, 2003·Journal of Neurophysiology·Tiziana SaccoFilippo Tempia
Nov 18, 2000·American Journal of Physiology. Cell Physiology·A CzarneckiL Bresson-Bepoldin
Jun 8, 2001·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Jürgen R. Schwarz, Christiane K. Bauer
Aug 24, 2002·The European Journal of Neuroscience·Andrea BecchettiEnzo Wanke
Sep 1, 1996·Pflügers Archiv : European journal of physiology·C K BauerJ R Schwarz
Jul 6, 2004·The Journal of Physiology·Wiebke HirdesBertil Hille
Apr 12, 2003·Pflügers Archiv : European journal of physiology·Jun Hee KimSung Joon Kim
Apr 20, 2004·Journal of Cellular and Molecular Medicine·Jürgen R Schwarz, Christiane K Bauer
Aug 1, 1994·Pflügers Archiv : European journal of physiology·C K BauerW T Mason
Oct 1, 1993·The American Journal of Physiology·M Inoue, I Imanaga

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