An ionotropic GABA receptor in cultured mushroom body Kenyon cells of the honeybee and its modulation by intracellular calcium

Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology
Bernd Grünewald, Anna Wersing

Abstract

GABAergic inhibitory transmission is very abundant within the insect brain. We, therefore, studied the functional properties of the ionotropic GABA receptor of honeybee mushroom body Kenyon cells in vitro. GABA applications elicit rapidly activating and desensitizing currents, which are concentration-dependent between 10 and 500 microM. The mean peak amplitude induced by 500 microM GABA at a holding potential of -110 mV is -1.55 +/- 0.23 nA (SEM, n = 29). The GABA-induced current is mediated by Cl(-) ions because (1) the reversal potential of the GABA-induced current of -40.6 mV is very close to the calculated Nernst potential of chloride (-44.8 mV). (2) With equimolar chloride concentrations the reversal potential shifted to about 0 mV. GABA or muscimol are equally efficient channel agonists, whereas CACA is a partial agonist. Picrotoxin or philanthotoxin (100 microM) completely and reversibly block the GABA-induced current, bicuculline (100 microM) has no effect. Elevating the intracellular Ca(2+) concentration increases the GABA current amplitude. This modulatory effect is blocked by the kinase blocker K 252a, but not by blockers of CaMkinaseII (KN-93), PKC (bisindolylmaleimide) or PKA (KT 5720). We conclude that Kenyon cell...Continue Reading

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Citations

Dec 24, 2013·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Anja FroeseRandolf Menzel
Aug 23, 2012·Pharmacology, Biochemistry, and Behavior·Katia BoumgharCatherine Armengaud
Jan 23, 2016·Journal of Immunology Research·Laura M Brutscher, Michelle L Flenniken
Aug 30, 2008·Neurobiology of Learning and Memory·Julie A MustardGeraldine A Wright
Jun 26, 2009·Journal of Neurophysiology·Heike Demmer, Peter Kloppenburg
Jul 18, 2014·Journal of Neurophysiology·Mary J Palmer, Jenni Harvey

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