PMID: 2463235Jan 1, 1988

An isoelectricfocusing method to detect hemoglobin variants in newborn blood samples including the beta-thalassemias

Hemoglobin
J Black

Abstract

An isoelectricfocusing method has been developed to enable large scale screening of newborn blood for hemoglobinopathies. The method utilizes a thin layer agarose gel containing carrier ampholytes in the pH range 6-8. The gradient is non linear allowing increased resolution in the pH region 6.8-7.8 where most hemoglobin variants are isoelectric. The increased resolution between Hb A and Hb Fac enables screening for beta-thalassemia by densitometric analysis. By calculating the ratio of Hb A/Hb Fac or Hb F/Hb A it is possible to detect newborns heterozygous for beta(+)- or beta(0)-thalassemia. Structural variants, unresolved by other electrophoretic methods, are easily detected. Seventy-two samples can be analyzed on a single gel in 90 minutes. Traces of hemoglobin variants can be detected by a heme-specific stain requiring no destain step.

Citations

Feb 20, 2004·Molecular Genetics and Metabolism·Zhili LinEdwin W Naylor
May 1, 1995·Hemoglobin·M A RosenthalN Epstein
Oct 25, 2012·Journal of Medical Screening·Fleur WolffBéatrice Gulbis
Dec 3, 2014·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Sai Krishna TikkaMohammad Zia Ul Haq Katshu
Sep 18, 1999·Hemoglobin·M M MarwanA E Felice

Related Concepts

Umbilical Cord Blood
Fixatives
Eryhem
Isoelectric Focusing
Staining and Labeling
Thalassemia

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