PMID: 9166418Jun 2, 1997Paper

An isotype-specific activator of major histocompatibility complex (MHC) class II genes that is independent of class II transactivator

The Journal of Experimental Medicine
J DouhanL H Glimcher

Abstract

Patients with one type of major histocompatibility complex class II combined immunodeficiency have mutations in a gene termed class II transactivator (CIITA), which coordinately controls the transcription of the three major human class II genes, HLA-DR, -DQ, and -DP. However, the experimentally derived B-lymphoblastoid cell line, clone 13, expresses high levels of HLADQ in the absence of HLA-DR and HLA-DP, despite its mapping by complementation analysis to this group. It was possible that one of the clone 13 CIITA alleles bore a mutation that allowed HLA-DQ, but not HLA-DR or -DP transcription. Alternatively, another factor, distinct from CIITA, might control HLA-DQ expression. We report here that ectopic expression of CIITA cDNAs derived by reverse transcriptase polymerase chain reaction from clone 13 do not restore expression of HLA-DQ in another CIITA-deficient cell line, RJ2.2.5. In addition, no CIITA protein is detectable in clone 13 nuclear extracts. In contrast, somatic cell fusion between clone 13 and RJ2.2.5 restored expression of the HLA-DQ haplotype encoded by the RJ2.2.5 DQB gene. Taken together, these data demonstrate the existence of an HLA-DQ isotype-specific trans-acting factor, which functions independently of ...Continue Reading

References

Jan 1, 1992·Annual Review of Immunology·L H Glimcher, C J Kara
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B Bénichou, J L Strominger
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S J OnoL H Glimcher
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D R LuceyP F Weller
Jan 1, 1986·Advances in Immunology·R H Schwartz
Oct 30, 1971·Lancet·M Seabright
Sep 1, 1995·International Immunology·C H ChangR A Flavell
Jan 1, 1995·Immunogenetics·A PeijnenburgP J van den Elsen
Feb 1, 1996·International Immunology·J DouhanL H Glimcher
Jan 1, 1996·Annual Review of Immunology·B MachW Reith

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Citations

Dec 30, 1999·Microbes and Infection·C Alcaïde-LoridanM Fellous
Dec 30, 1999·Microbes and Infection·J S BeatyG T Nepom
Dec 30, 1999·Microbes and Infection·T N Sims, P F Halloran
Dec 30, 1999·Microbes and Infection·J D FontesB M Peterlin
Mar 30, 2004·Journal of Virology·Barry SlobedmanEdward S Mocarski
Aug 11, 2000·Molecular and Cellular Biology·J A Harton, J P Ting
Mar 13, 2001·Annual Review of Immunology·W Reith, B Mach
Jan 25, 2003·Tissue Antigens·M T CoirasM Sánchez-Pérez
Nov 25, 2003·Clinical and Experimental Immunology·L E ReesM Bailey
Aug 11, 2004·Virology·Patricia M A de GraaffGrada M van Bleek
Jun 17, 2010·Journal of Leukocyte Biology·Charlotte F InmanMick Bailey
Dec 20, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·W J MagnerT B Tomasi
Nov 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wiltrud M Kalka-MollDennis L Kasper
Jan 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paula R MengesAndrea J Sant
Apr 18, 2018·International Journal of Immunogenetics·M ZajacovaM Cerna

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