PMID: 8454576Jan 1, 1993Paper

An isozyme of microsomal carboxyesterases, carboxyesterase Sec, is secreted from rat liver into the blood

Journal of Biochemistry
K MurakamiT Omura

Abstract

It is generally believed that liver carboxyesterases are localized exclusively in the endoplasmic reticulum (ER), mostly in the lumen, loosely bound to the inner side of the membrane. A cDNA clone, clone (8-1/2-1) supposed to code for one of the isozymes, carboxyesterase E1, was isolated by Takagi et al. [J. Biochem. 104, 801-806 (1988)]. However, the protein coded by clone (8-1/2-1) had no consensus ER retention signal at its carboxy terminus, and the mechanism of its retention by ER lumen was unclear. When clone (8-1/2-1) was expressed in COS cells in this study, the plasmid-coded protein was secreted into the medium. When the carboxy terminal portion of the clone (8-1/2-1)-coded protein was replaced with the corresponding region of another carboxyesterase, pI 6.1 esterase, which had the HVEL sequence at the carboxy terminus, the chimeric protein was retained in the COS cells. We searched for a secretory form carboxyesterase in rat blood immunochemically using polyclonal antibodies to carboxyesterase E1, and detected a cross-reacting protein with a molecular weight of 68 kDa. The molecular weight was decreased by endoglycosidase F treatment but not by endoglycosidase H treatment, indicating that the protein carries complex ty...Continue Reading

Citations

Feb 20, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Shiori TakahashiTsuyoshi Yokoi
May 23, 1998·Annual Review of Pharmacology and Toxicology·T Satoh, M Hosokawa
Aug 11, 1995·The Journal of Biological Chemistry·B YanA Parkinson
Feb 27, 1999·Antimicrobial Agents and Chemotherapy·C D Sohaskey, A G Barbour
Jul 9, 1999·Biochimica Et Biophysica Acta·M A DiczfalusyS E Alexson

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