PMID: 8952955Dec 1, 1996Paper

An L-type calcium channel in renal epithelial cells

The Journal of Membrane Biology
M I Zhang, R G O'Neil

Abstract

A voltage-activated Ca++ channel has been identified in the apical membranes of cultured rabbit proximal tubule cells using the patch-clamp technique. With 105 mm CaCl2 solution in the pipette and 180 NaAsp in the bath, the channel had a conductance of 10.4 +/- 1.0 pS (n = 8) in on-cell patches, and 9.8 +/- 1.1 pS (n = 8) in inside-out patches. In both on-cell and inside-out patches, the channel is active by membrane depolarization. For this channel, the permeation to Ba++ and Ca++ is highly selective over Na+ and K+ (PCa(Ba):PNa(K) >200:1). The sensitivity to dihydropyridines is similar to that for L-type channels where the channel was blocked by nifedipine (10 microM), and activated by Bay K 8644 (5 microM). When activated by Bay K 8644, the channel showed subconductance levels. Treatment with forskolin (12.5 microM), phorbol ester (1 microM), or stretching (40 cm water) did not activate this channel. These results indicate that this Ca++ channel is mostly regulated by membrane voltage, and appears to be an epithelial class of L-type Ca++ channel. As such, it may participate in calcium reabsorption during periods of enhanced sodium reabsorption, or calcium signaling in volume regulation, where membrane depolarization occurs f...Continue Reading

Citations

Oct 21, 1999·Journal of Cellular Physiology·M G BrunetteG Hilal
Jan 20, 1998·Drug and Chemical Toxicology·G L DiamondW L Ruoff
May 23, 1998·Drug and Chemical Toxicology·G L DiamondW L Ruoff
Dec 15, 2005·Zoology : Analysis of Complex Systems, ZACS·Gerald S Braun, Stephan M Huber
Apr 6, 2016·Channels·Evgeny Kobrinsky, Nikolai M Soldatov
Mar 31, 2004·Canadian Journal of Physiology and Pharmacology·M G BrunetteY Bourgeois
May 17, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·W WangS Nielsen
Jul 5, 2003·Physiological Reviews·Julian T Dow, Shireen A Davies
Feb 8, 2000·American Journal of Physiology. Renal Physiology·D A HallS C Sansom
Jun 16, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·J R LatendresseK B Delclos
Aug 8, 1997·Biochemical and Biophysical Research Communications·R G O'NeilN J Karin

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