An LC-MS/MS method for determination of forsythiaside in rat plasma and application to a pharmacokinetic study

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Geng-Nan WangQi Chang

Abstract

A highly sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed for the determination of forsythiaside in rat plasma using epicatechin as internal standard. The analytes were extracted by solid-phase extraction and chromatographied on a C(18) column eluted with a gradient mobile phase of acetonitrile and water both containing 0.2% formic acid. The detection was performed by negative ion electrospray ionization in multiple reaction monitoring mode, monitoring the transitions m/z 623-->161 and m/z 289-->109 for forsythiaside and epicatechin, respectively. The assay was linear over the concentration ranges of 2.0-50.0 and 50.0-5000.0ng/mL with limits of detection and quantification of 0.2 and 1.0ng/mL, respectively. The precision was <10.8% and the accuracy was >91.9%, and extraction recovery ranged from 81.3% to 85.0%. This method was successfully applied to a pharmacokinetic study of forsythiaside in rats after intravenous (20mg/kg) and oral (100mg/kg) administration, and the result showed that the compound was poorly absorbed with an absolute bioavailability being approximately 0.5%.

References

Feb 3, 2005·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Toru Iizuka, Masahiro Nagai

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Citations

Feb 9, 2012·European Journal of Drug Metabolism and Pharmacokinetics·Yang ChuYonghong Zhu
Mar 21, 2012·European Journal of Drug Metabolism and Pharmacokinetics·Yun-Xia LiCheng Peng
Dec 3, 2014·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Wei ZhouLiuqing Di
Nov 21, 2015·Journal of Traditional and Complementary Medicine·Piyush MehtaK R Mahadik
May 20, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yang BaiYan-xu Chang
Oct 24, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Wei ZhouLiu Qing Di
Aug 3, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhenzhen Xue, Bin Yang
Oct 19, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu-Tse WuMei-Chich Hsu
Sep 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhanglu DongMenghua Liu
Nov 22, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Xia WangLing Yang
Aug 12, 2020·Medicinal Research Reviews·Lipeng WuBaiyi Lu
May 25, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Lihong GongYunxia Li

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