An LC/MS quantitative and microdialysis method for cyclovirobuxine D pharmacokinetics in rat plasma and brain: The pharmacokinetic comparison of three different drug delivery routes

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Jia-Bao WeiYang Yu

Abstract

To explore the brain-targeting of cyclovirobuxine D(CVB-D) after administered intranasally, the pharmacokinetics of CVB-D via three different drug delivery routes: intragastric (i.g.), intranasal (i.n.), and intravenous (i.v.) in rat brain and blood was compared. Firstly, an in vivo microdialysis method for sampling CVB-D in both plasma and brain of the rat was established. Secondly, a liquid chromatography-tandem mass spectrometry method has been developed and validated for determination of CVB-D in microdialysis samples. For plasma and brain microdialysis samples, liquid-liquid extraction was used and donepezil was chosen as internal standard. Both were followed by HPLC separation and positive electrospray ionization tandem mass spectrometry detection (ESI-MS/MS). Chromatographic separation was achieved on a agilent C18 column with a mobile phase of methanol-water (50:50, v/v) (pH 3.2) containing 0.1% formic acid and 5mM ammonium acetate. Mass spectrometric detection in the positive ion mode was carried out by selected reaction monitoring (MRM) of the transitions at m/z 403.4→372.3 for CVB-D and m/z 380.2→243.1 for donepezil (IS). Good linearities were obtained in the range of 10-4000ng/mL in rat microdialysates for CVB-D. Th...Continue Reading

References

Apr 29, 2005·Journal of Pharmaceutical Sciences·Candace L Graff, Gary M Pollack
Jun 22, 2005·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Elena GrossiniGiorgio Bellomo
Nov 25, 2005·Current Drug Delivery·Tushar K VyasAmbikanandan Misra
Jun 13, 2006·Journal of Pharmaceutical and Biomedical Analysis·Peng YuQian Yang
Jun 8, 2007·Pharmaceutical Research·Ruben J Boado
May 6, 2008·International Journal of Pharmaceutics·Mukesh KumarKamla Pathak
Aug 8, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Xiaomei WangXing Tang
Mar 3, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ziyi WenRen Lai
Aug 9, 2011·International Journal of Pharmaceutics·Adriana TrapaniGiuseppe Trapani
Aug 30, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·William M Pardridge
Mar 8, 2013·Journal of Pharmacokinetics and Pharmacodynamics·Richard J ShannonPeter J Hutchinson
May 8, 2013·The Journal of Pharmacy and Pharmacology·Xi ChenYilin Yang
Sep 12, 2013·Cell and Tissue Research·Elmira Anderzhanova, Carsten T Wotjak
Oct 17, 2013·Drug Delivery·Hitendra S MahajanAnshuman Agrawal
Nov 20, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·André SchaeftleinCharlotte Kloft
May 13, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Ana SerralheiroAmílcar Falcão

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Citations

May 18, 2016·Molecular Neurobiology·Chun Min KhoNorazharuddin Shah Abdullah
Oct 18, 2016·Journal of Mass Spectrometry : JMS·Laszlo ProkaiMichael J Forster
Nov 9, 2016·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Yinai WuLisheng Wang
Jul 21, 2020·Critical Reviews in Analytical Chemistry·Akhila PyaramPinaki Sengupta
Dec 9, 2016·European Journal of Drug Metabolism and Pharmacokinetics·Pengyue LiPuning Cao

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