PMID: 9653122Jul 8, 1998Paper

An N-terminal fragment of the gene 4 helicase/primase of bacteriophage T7 retains primase activity in the absence of helicase activity

Proceedings of the National Academy of Sciences of the United States of America
D N FrickCharles C Richardson

Abstract

Primase and helicase activities of bacteriophage T7 are present in a single polypeptide coded by gene 4. Because the amino terminal region of the gene 4 protein contributes to primase activity, we constructed a truncated gene 4 encoding the N-terminal 271-aa residues. The truncated protein, purified from cells overexpressing the protein, is a dimer in solution; the full-length protein is a hexamer. Although the fragment is devoid of dTTPase and helicase activities, it catalyzes template-directed synthesis of di-, tri-, and tetranucleotides. The rates for tetraribonucleotide synthesis and for dinucleotide extension on a 20-nucleotide template are similar for the full-length and truncated proteins. However, the activity of the primase fragment is unaffected by dTTP whereas the primase activity of the full-length protein is stimulated >14-fold. The primase fragment is defective in the interaction with T7 DNA polymerase in that primer synthesis cannot be coupled to DNA synthesis.

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Citations

May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Bin ZhuCharles C Richardson
May 31, 2012·Proceedings of the National Academy of Sciences of the United States of America·Arkadiusz W Kulczyk, Charles C Richardson
May 19, 2012·The Journal of Biological Chemistry·Huidong ZhangCharles C Richardson
Aug 22, 2007·Nucleic Acids Research·Kirsten Beck, Georg Lipps
Jun 19, 2008·Nucleic Acids Research·Ying LiHuimin Kong
Mar 31, 2010·Nucleic Acids Research·Seung-Joo LeeCharles C Richardson
Jul 2, 2010·Nucleic Acids Research·Marilynn A LarsonSteven H Hinrichs
May 22, 2002·Protein Science : a Publication of the Protein Society·Yanshun Liu, David Eisenberg
Jun 8, 2001·Annual Review of Biochemistry·S J BenkovicF Salinas
Jun 8, 2001·Annual Review of Biochemistry·D N Frick, C C Richardson
Mar 21, 2009·Annual Review of Biochemistry·Samir M Hamdan, Charles C Richardson
May 14, 2010·Annual Review of Biophysics·Antoine M van Oijen, Joseph J Loparo
Sep 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Seung-Joo Lee, Charles C Richardson
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·K ChowdhuryC C Richardson
Mar 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Samir M HamdanCharles C Richardson
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·T KusakabeC C Richardson
Dec 1, 2015·Critical Reviews in Biochemistry and Molecular Biology·Laurie S Kaguni, Marcos T Oliveira
Feb 28, 2007·Journal of Molecular Biology·Tawn D ZiebarthLaurie S Kaguni
Feb 24, 2006·Journal of Molecular Biology·Rafael Núñez-RamírezLuis Enrique Donate
Jun 4, 2015·Annual Review of Biochemistry·Charles C Richardson
Nov 26, 2003·Molecular Cell·Eric A TothTom Ellenberger
Dec 17, 2008·Biochimica Et Biophysica Acta·Teresa HolmlundMaria Falkenberg
Jun 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Seung-Joo LeeCharles C Richardson
May 11, 2016·Proceedings of the National Academy of Sciences of the United States of America·Alfredo J HernandezCharles C Richardson
Jan 25, 2007·Molecular BioSystems·Antoine M van Oijen
Feb 3, 2006·Nature·Jong-Bong LeeAntoine M van Oijen
May 27, 2005·The Journal of Biological Chemistry·Seung-Joo Lee, Charles C Richardson
Dec 10, 1999·The Journal of Biological Chemistry·D N Frick, C C Richardson
Dec 10, 1999·The Journal of Biological Chemistry·D N FrickC C Richardson
Oct 24, 2001·The Journal of Biological Chemistry·S J Lee, C C Richardson
May 11, 2004·The Journal of Biological Chemistry·Masato KatoTom Ellenberger
Mar 30, 2001·The Journal of Biological Chemistry·M KatoT Ellenberger
Sep 23, 2003·The Journal of Biological Chemistry·Michael A TrakselisStephen J Benkovic

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