An N-terminally acetylated Arf-like GTPase is localised to lysosomes and affects their motility

Journal of Cell Science
Irmgard Hofmann, Sean Munro

Abstract

Small GTPases of the Arf and Rab families play key roles in the function of subcellular organelles. Each GTPase is usually found on only one compartment and, hence, they confer organelle specificity to many intracellular processes. However, there has so far been little evidence for specific GTPases present on lysosomes. Here, we report that two closely related human Arf-like GTPases, Arl8a and Arl8b (also known as Arl10b/c and Gie1/2), localise to lysosomes in mammalian cells, with the single homologue in Drosophila cells having a similar location. Conventionally, membrane binding of Arf and Arl proteins is mediated by both an N-terminal myristoyl group and an N-terminal amphipathic helix that is inserted into the lipid bilayer upon activation of the GTPase. Arl8a and Arl8b do not have N-terminal myristoylation sites, and we find that Arl8b is instead N-terminally acetylated, and an acetylated methionine is necessary for its lysosomal localization. Overexpression of Arl8a or Arl8b results in a microtubule-dependent redistribution of lysosomes towards the cell periphery. Live cell imaging shows that lysosomes move more frequently both toward and away from the cell periphery, suggesting a role for Arl8a and Arl8b as positive regu...Continue Reading

References

Jul 1, 1985·The Journal of Cell Biology·J W ChenJ T August
Sep 1, 1987·The Journal of Cell Biology·R Matteoni, T E Kreis
Feb 9, 1996·Cell·G Warren, W Wickner
Sep 27, 1996·The Journal of Biological Chemistry·H StenmarkA D'Arrigo
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·A ShevchenkoM Mann
May 16, 1998·The Journal of Cell Biology·A HaradaN Hirokawa
Aug 11, 1998·Trends in Biochemical Sciences·R A BradshawK W Walker
Mar 8, 2000·The Journal of Cell Biology·S P GrossE F Wieschaus
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·M Zerial, H McBride
Nov 24, 2001·Trends in Cell Biology·S R Pfeffer
Apr 17, 2002·Journal of Molecular Biology·Sebastian Maurer-StrohFrank Eisenhaber
Jul 27, 2002·Annual Review of Cell and Developmental Biology·James Shorter, Graham Warren
Jan 1, 2003·Journal of Molecular Biology·Bogdan Polevoda, Fred Sherman
Mar 7, 2003·Current Biology : CB·Subba Rao Gangi SettyChristopher G Burd
May 14, 2003·The EMBO Journal·Simon L BullockDavid Ish-Horowicz
Aug 2, 2003·Current Opinion in Cell Biology·Zhongzhen NiePaul A Randazzo
Aug 20, 2003·The Journal of Cell Biology·Yoshikatsu Aikawa, Thomas F J Martin
Oct 31, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nihal Altan-BonnetJennifer Lippincott-Schwartz
May 1, 2004·Traffic·Miguel C Seabra, Evelyne Coudrier
Jun 2, 2004·Nature Cell Biology·Sean Munro
Jun 11, 2004·Annual Review of Biochemistry·Jessica E Smotrys, Maurine E Linder
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Marcus C S LeeRandy Schekman
Oct 12, 2004·Annual Review of Cell and Developmental Biology·David PruyneAnthony Bretscher
Nov 24, 2004·Current Biology : CB·Roop Mallik, Steven P Gross
Nov 27, 2004·Trends in Cell Biology·Christopher G BurdSubba R Gangi Setty
Dec 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yawei LiRichard A Kahn
Feb 23, 2005·Current Biology : CB·Nicholas A BrightJ Paul Luzio
Jun 28, 2005·Biochimica Et Biophysica Acta·Julie G DonaldsonRoberto Weigert
Jul 27, 2005·Biochemical Society Transactions·S Munro
Sep 1, 2005·Journal of Cell Science·Hye-Won ShinKazuhisa Nakayama
Dec 2, 2005·Nature·Rudy Behnia, Sean Munro
Mar 1, 2006·The Journal of Cell Biology·Richard A KahnAnnette Schurmann

❮ Previous
Next ❯

Citations

Apr 5, 2011·Nature Cell Biology·Christian Poüs, Patrice Codogno
Mar 12, 2011·Nature Cell Biology·Viktor I KorolchukDavid C Rubinsztein
May 19, 2011·Nature Reviews. Molecular Cell Biology·Julie G Donaldson, Catherine L Jackson
Mar 7, 2012·Oncogene·T V Kalvik, T Arnesen
Jan 15, 2011·The Journal of Biological Chemistry·Yong-Kang YangDan-Ying Chen
Jan 22, 2011·Molecular & Cellular Proteomics : MCP·Maria Cecilia Della VallePeter Lobel
Oct 10, 2009·Molecular & Cellular Proteomics : MCP·Bai-Yu LeeMarcus A Horwitz
Oct 4, 2013·Molecular Biology of the Cell·Amit TuliMichael B Brenner
May 18, 2007·Annual Review of Cell and Developmental Biology·Alison K Gillingham, Sean Munro
Aug 8, 2009·BMC Proceedings·Kristian K StarheimThomas Arnesen
Dec 17, 2011·PLoS Pathogens·Masaki NishikioriMasayuki Ishikawa
May 21, 2014·The Journal of Cell Biology·Natalia SchiefermeierLukas A Huber
Feb 19, 2013·Journal of Cell Science·Jahan YousefianThomas Klein
Feb 11, 2016·Scientific Reports·Om Singh RathoreRui Gonçalo Martinho
Aug 21, 2012·Traffic·Mary-Pat SteinAngela Wandinger-Ness
Aug 23, 2012·Traffic·Amra MrakovicRoberto J Botelho
Mar 13, 2012·Trends in Biochemical Sciences·Kristian K StarheimThomas Arnesen
Dec 11, 2008·Journal of Proteomics·John W CallahanDon J Mahuran
Sep 4, 2007·Trends in Cell Biology·Michael A Welte
Apr 9, 2016·Cellular Logistics·Divya KhatterMahak Sharma
Sep 28, 2007·Traffic·Bernd SchröderAndrej Hasilik
Mar 16, 2016·The Journal of Cell Biology·Danielle E JohnsonSergio Grinstein
Jan 19, 2008·Proteomics·Thierry Meinnel, Carmela Giglione
Jul 9, 2011·The FEBS Journal·Petra Van DammeKris Gevaert
Jun 3, 2011·Protein Science : a Publication of the Protein Society·Alexander Varshavsky
Oct 20, 2010·Proteomics·Bernd A SchröderPaul Saftig
Mar 20, 2015·Cell Cycle·Marta PortelaHelena E Richardson
May 3, 2006·Biochemical and Biophysical Research Communications·Richard D BagshawDon J Mahuran
Apr 30, 2015·Proteomics·Rui D Silva, Rui G Martinho
Oct 1, 2011·Developmental Biology·Bo ZhouXinhua Lin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.