An NBD Derivative of the Selective Rat Toxicant Norbormide as a New Probe for Living Cell Imaging

Frontiers in Pharmacology
Claudio D'AmoreSergio Bova


Norbormide (NRB) is a unique compound that acts directly on rat vascular myocytes to trigger a contractile process, through an as yet unknown mechanism, which results in the selective contraction of rat peripheral arteries. To gain insight into the mechanisms involved in NRB rat-selective activity, we investigated the subcellular distribution of NRB-AF12, a nitrobenzoxadiazole (NBD)-derivative of NRB, in living NRB-sensitive and NRB-insensitive cells. In both cell types, NRB-AF12 localized to the endoplasmic reticulum (ER), Golgi apparatus, mitochondria, lysosomes, and endosomes; however, in NRB-sensitive cells, the fluorescence also extended to the plasma membrane. NRB-AF12 was rapidly internalized into the cells, could easily be washed out and then reloaded back into the same cells, all with a high degree of reproducibility. Cells exposed for 24 h to NRB-AF12 did not show apparent signs of toxicity, even at concentrations of the dye (10 μM) much higher than those required for fluorescence labeling (500 ηM). The distribution pattern of NRB-AF12 fluorescence was near identical to that of ER-Tracker® (Er-Tr), a fluorescent derivative of glibenclamide, a known KATP channel blocker. Displacement tests did not demonstrate, but at t...Continue Reading


Jul 1, 1966·Journal of Medicinal Chemistry·G I PoosA P Roszkowski
Feb 12, 1998·The American Journal of Physiology·H YokoshikiN Sperelakis
Dec 12, 2002·Diabetes·Peter ProksFrances Ashcroft
Feb 1, 1965·The Journal of Pharmacy and Pharmacology·E G CLARKE
Jun 15, 2005·Biochimica Et Biophysica Acta·Fernanda RicchelliSergio Bova
Jun 28, 2005·Journal of Molecular and Cellular Cardiology·Nian-Qing ShiJonathan C Makielski
Feb 18, 2006·The Journal of Physiology·Noriyoshi Teramoto
May 19, 2007·Biochimica Et Biophysica Acta·Alessandra ZulianFernanda Ricchelli
Jun 17, 2008·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Eun A KoWon Sun Park
Mar 23, 2010·The Journal of Membrane Biology·Keat-Eng NgAndrew Tinker
Oct 26, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Li BaoMichael J Rindler
Oct 10, 2013·British Journal of Pharmacology·Andrew TinkerAlison Thomas

❮ Previous
Next ❯


Dec 7, 2018·Molecular Diversity·Xuelian LiuZhaohai Qin
Dec 6, 2019·Frontiers in Neuroscience·Barbara NapoliGenny Orso

❮ Previous
Next ❯

Datasets Mentioned


Methods Mentioned

confocal microscopy
column chromatography

Software Mentioned

NIS Elements

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Pharmacology and Experimental Therapeutics
A P Roszkowski
Bioorganic & Medicinal Chemistry Letters
Rika YamadaKaori Sakurai
© 2021 Meta ULC. All rights reserved