An O antigen capsule modulates bacterial pathogenesis in Shigella sonnei

PLoS Pathogens
Mariaelena CaboniChristiane Gerke

Abstract

Shigella is the leading cause for dysentery worldwide. Together with several virulence factors employed for invasion, the presence and length of the O antigen (OAg) of the lipopolysaccharide (LPS) plays a key role in pathogenesis. S. flexneri 2a has a bimodal OAg chain length distribution regulated in a growth-dependent manner, whereas S. sonnei LPS comprises a monomodal OAg. Here we reveal that S. sonnei, but not S. flexneri 2a, possesses a high molecular weight, immunogenic group 4 capsule, characterized by structural similarity to LPS OAg. We found that a galU mutant of S. sonnei, that is unable to produce a complete LPS with OAg attached, can still assemble OAg material on the cell surface, but a galU mutant of S. flexneri 2a cannot. High molecular weight material not linked to the LPS was purified from S. sonnei and confirmed by NMR to contain the specific sugars of the S. sonnei OAg. Deletion of genes homologous to the group 4 capsule synthesis cluster, previously described in Escherichia coli, abolished the generation of the high molecular weight OAg material. This OAg capsule strongly affects the virulence of S. sonnei. Uncapsulated knockout bacteria were highly invasive in vitro and strongly inflammatory in the rabbit ...Continue Reading

References

Mar 1, 1991·Reviews of Infectious Diseases·M L Bennish
Mar 1, 1990·The Journal of Infectious Diseases·M L BennishM Struelens
Jul 1, 1988·Journal of General Microbiology·N OkamuraY Matsubara
Oct 1, 1985·The Journal of Infectious Diseases·M J StruelensT Butler
May 1, 1970·European Journal of Biochemistry·W DrögeO Westphal
Mar 1, 1970·The Journal of Infectious Diseases·K C HaltalinA A Allen
Jan 1, 1967·Proceedings of the Society for Experimental Biology and Medicine·S B FormalS Berman
Oct 1, 1981·Infection and Immunity·P J SansonettiS B Formal
Feb 1, 1981·American Journal of Diseases of Children·B DuncanK J Ryan
Jan 1, 1996·Annual Review of Microbiology·I S Roberts
Feb 1, 1997·Molecular Microbiology·L Van den BoschR Morona
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Nov 10, 2000·Nucleic Acids Research·M K ChaverocheC d'Enfert
Feb 20, 2004·Journal of Bacteriology·Mark A SchembriPer Klemm
Feb 26, 2005·Science·Nicholas P WestChristoph M Tang
Jul 21, 2005·Journal of Bacteriology·Adi PelegIlan Rosenshine
Jul 26, 2005·Infection and Immunity·Mark A SchembriPer Klemm
Jun 8, 2006·Annual Review of Biochemistry·Chris Whitfield
Jul 22, 2006·Carbohydrate Research·David Scott SnyderParastoo Azadi
Jun 15, 2007·Nature Reviews. Microbiology·Myron M LevineMarcelo B Sztein
Apr 22, 2008·FEMS Microbiology Reviews·Bin LiuLei Wang

❮ Previous
Next ❯

Citations

Mar 1, 2016·Nature Reviews. Microbiology·Hao Chung TheStephen Baker
Nov 8, 2016·Nature Microbiology·Gareth McVicker, Christoph M Tang
Jun 16, 2017·Cell Host & Microbe·Mark C AndersonPhilippe J Sansonetti
Feb 25, 2018·Applied and Environmental Microbiology·Jayne WatsonAbigail Clements
Jul 27, 2018·Current Opinion in Infectious Diseases·Stephen Baker, Hao Chung The
Apr 17, 2019·Infection and Immunity·Volkan K KöseoğluHervé Agaisse
Nov 30, 2019·FEMS Microbiology Reviews·Bin LiuGöran Widmalm
Jul 31, 2018·Genome Biology and Evolution·Anna Allué-GuardiaMark Eppinger
Sep 16, 2020·Archives of Microbiology·Ahtesham Ahmad Shad, Wajahat Ahmed Shad
May 1, 2020·Frontiers in Immunology·Samantha A Hsieh, Paul M Allen
Jan 17, 2021·Genome Medicine·Ruklanthi de AlwisStephen Baker
Jan 15, 2021·Proceedings of the National Academy of Sciences of the United States of America·Christopher A Caffalette, Jochen Zimmer
Dec 15, 2020·Chemistry : a European Journal·Debashis Dhara, Laurence A Mulard
Nov 16, 2021·PloS One·Matthew R LarsonMark A Saper

❮ Previous
Next ❯

Methods Mentioned

BETA
glucosylation
flow cytometry
electron microscopy
Fluorescence
PCR
Assay
Protein Assay
Electrophoresis
biopsies

Software Mentioned

FlowJo
pSS
Artemis browser
Mestrenova

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.