An on-line high-performance liquid chromatography-diode-array detector-electrospray ionization-ion-trap-time-of-flight-mass spectrometry-total antioxidant capacity detection system applying two antioxidant methods for activity evaluation of the edible flowers from Prunus mume

Journal of Chromatography. a
Xiaoxia ZhangHong Wang

Abstract

An on-line high-performance liquid chromatography-diode-array detector-electrospray ionization-ion-trap-time-of-flight-mass spectrometry-total antioxidant capacity detection (HPLC-DAD-ESI-IT-TOF-MS-TACD) system was created for identification and evaluation of antioxidants in Prunus (P.) mume flowers. Applying this system, the HPLC fingerprint, ultraviolet (UV) spectra, mass fragmentations, active profiles against 1,1-diphenylpicryl-2-hydrazyl radical (DPPH•) scavenging activity and ferric reducing antioxidant power (FRAP) of each complex sample were obtained simultaneously after one injection. Synchronous structure identification and activities screening of complex samples were thus accomplished. In this study, 78 compounds were identified from P. mume flowers by their chromatographic behaviors, UV spectra and MS data with the assistance of standard compounds and literature reports. The DPPH and FRAP activity of 24 samples (23 different P. mume varieties and 1 related herbal medicine) were then quantified by their detailed activity profiles from the on-line system, and by the total activity of each sample extract from off-line 96-well plate method. As a result, 21 and 32 compounds in the on-line system showed anti-oxidative eff...Continue Reading

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Citations

Apr 19, 2018·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Hengqiang ZhaoXiao Wang
Jul 5, 2017·Journal of the Science of Food and Agriculture·Emilie StierlinThomas Michel
Jul 8, 2020·Chemistry & Biodiversity·Vanja Ljoljić BilićJadranka Vuković Rodriguez
Jan 18, 2018·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Minmin ZhangHengqiang Zhao
Nov 30, 2019·Biomolecules·Mirjana MosićDušanka Milojković-Opsenica
Jul 26, 2017·Journal of Chromatography. a·Hong WangZongtao Lin
May 11, 2021·Analytical Methods : Advancing Methods and Applications·Weifeng FanZhengming Qian

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