An On-Site Simultaneous Semi-quantification of Aflatoxin B1, Zearalenone, and T-2 Toxin in Maize- and Cereal-based Feed via Multicolor Immunochromatographic Assay

Toxins
Lin XuPeiwu Li

Abstract

Multiple-mycotoxin contamination has been frequently found in the agro-food monitoring due to the coexistence of fungi. However, many determination methods focused on a single mycotoxin, highlighting the demand for on-site determination of multiple mycotoxins in a single run. We develop a multicolor-based immunochromatographic strip (ICS) for simultaneous determination of aflatoxin B1 (AFB1), zearalenone (ZEN) and T-2 toxin in maize- and cereal-based animal feeds. The nanoparticles with different colors are conjugated with three monoclonal antibodies, which serve as the immunoassay probes. The decrease in color intensity is observed by the naked eyes, providing simultaneous quantification of three mycotoxins. The visible limits of detection for AFB1, ZEN and T-2 are estimated to be 0.5, 2, and 30 ng/mL, respectively. The cut-off values are 1, 10, and 50 ng/mL, respectively. Considerable specificity and stability are found using real samples. The results are in excellent agreement with those from high-performance liquid chromatography/tandem mass spectrometry. The multi-color ICS boasts sensitive and rapid visual differentiation and simultaneous semi-quantification of aflatoxin B1, zearalenone and T-2 toxin in maize- and cereal-...Continue Reading

References

Sep 4, 2004·Environmental Health Perspectives·Yunyun GongChristopher P Wild
Mar 29, 2008·Journal of Agricultural and Food Chemistry·Alexandra MolinelliRudolf Krska
Oct 11, 2012·Molecular Nutrition & Food Research·Franz BerthillerJoerg Stroka
Jun 19, 2013·Critical Reviews in Food Science and Nutrition·Stoycho D Stoev
Oct 25, 2014·Journal of Agricultural and Food Chemistry·Yaning SunGaiping Zhang

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Citations

Nov 26, 2020·Toxins·Christopher Pöhlmann, Thomas Elßner
Jan 21, 2021·Toxins·Lizbeth Martínez-MartínezRaúl Ortiz-Martínez
Dec 18, 2020·Comprehensive Reviews in Food Science and Food Safety·Jing WanJiajia Rao
May 7, 2021·Critical Reviews in Food Science and Nutrition·Liu WangXiahong Xu
May 15, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Xudong ZhaoZhixian Gao
Jan 25, 2020·Journal of Agricultural and Food Chemistry·Jia-Xiang YanQing-Hua He

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Methods Mentioned

BETA
enzyme linked immunosorbent assay
ELISA
protein assay

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