An Oncolytic Adenovirus Expressing SNORD44 and GAS5 Exhibits Antitumor Effect in Colorectal Cancer Cells

Human Gene Therapy
Sujing YuanLiang Chu

Abstract

SNORD44 is a C/D box small nucleolar RNA, and exhibits low expression in breast cancer and head and neck squamous cell carcinoma tissues. Its host gene is growth arrest specific transcript 5 (GAS5), which is a long noncoding RNA. GAS5 is downregulated in colorectal cancer (CRC), and overexpression of GAS5 suppresses cell proliferation. However, the function of SNORD44 in CRC remains largely unknown, and the application of SNORD44 combined with GAS5 in CRC treatment has not been reported. In this study, the expression levels of SNORD44 and GAS5 were measured in CRC tissues by quantitative RT-PCR. The correlation between SNORD44 and GAS5 was evaluated by Pearson correlation analysis. An oncolytic adenovirus expressing SNORD44 and GAS5 (SPDD-UG) was constructed. The biological effects of SPDD-UG were investigated in CRC cell line SW620 and LS174T in vitro and in xenografts. The synergistic effect of rapamycin and SPDD-UG was explored in SW620 and LS174T cells and tumors. We demonstrated that SNORD44 expression level was markedly decreased in CRC tissues and positively correlated with GAS5 expression. SPDD-UG significantly inhibited SW620 and LS174T cell growth and induced cell apoptosis. Intratumoral injection of SPDD-UG significa...Continue Reading

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Citations

Nov 7, 2019·Cancer Medicine·Huan YangShiming Yang
Feb 18, 2020·Histochemistry and Cell Biology·Xian-Ru XiaGuo-Lin Yuan
Jul 25, 2019·Frontiers in Oncology·Junnan LiangLiang Chu
Feb 21, 2019·Molecular Cancer·Jing YangXiawei Wei
Oct 22, 2020·BioMed Research International·Zhengxiang ZhangHao Li
Jan 20, 2021·Cancer Cell International·Xiaoling ShangXianrang Song
Dec 19, 2019·RNA Biology·Shengyun MaWendy Jia Men Huang
Jan 2, 2021·Critical Reviews in Oncology/hematology·Shokooh SalimimoghadamBehzad Baradaran
Aug 28, 2021·Biology·Jildou van der WerfNicholas Ian Fleming
Apr 12, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghaforui-Fard, Mohammad Taheri

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Methods Mentioned

BETA
PCR
flow cytometry
xenograft
xenografts

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