An open reading frame encoding a putative haem-binding polypeptide is cotranscribed with the pea chloroplast gene for apocytochrome f

Plant Molecular Biology
D L Willey, J C Gray

Abstract

The nucleotide sequence of a 1 kbp region of pea chloroplast DNA upstream from the gene petA encoding apocytochrome f has been determined. An open reading frame of 231 codons (ORF231) encoding a putative membrane-spanning polypeptide is separated by 205 bp from the coding region of petA. The open reading frame is homologous to open reading frames located in a similar position with respect to petA in chloroplast DNA from Marchantia polymorpha, tobacco, rice, wheat and Vicia faba. The sequence around a conserved histidine residue in a putative membrane-spanning region of the polypeptide resembles sequences present in cytochrome b from chromaffin granules and neutrophil membranes, suggesting that the open reading frame may encode a haem-binding polypeptide, possibly a b-type cytochrome. Northern hybridisation analysis indicates the presence in pea chloroplasts of a complex pattern of transcripts containing ORF231. Large transcripts of 5.5 kb, 4.3 kb, 3.4 kb and 2.7 kb encode both ORF231 and apocytochrome f, indicating that ORF231 and petA are co-transcribed.

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G K McMaster, G G Carmichael
Jan 2, 1979·European Journal of Biochemistry·W Bottomley, P R Whitfeld
Dec 1, 1975·Photochemistry and Photobiology·W A Cramer, P Horton
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·N H Chua, P Bennoun
Feb 1, 1988·European Journal of Biochemistry·P Westhoff, R G Herrmann
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J Haley, L Bogorad
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C A ParkosS H Orkin
Mar 11, 1987·Nucleic Acids Research·K Ko, N A Straus
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G HindS Izawa
May 15, 1973·FEBS Letters·J M Anderson, N K Boardman
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·K Wada, D I Arnon
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Oct 25, 1980·Journal of Molecular Biology·F SangerB A Roe
Apr 1, 1981·Analytical Biochemistry·G DretzenP Chambon
Jun 1, 1983·European Journal of Biochemistry·G von Heijne
Jun 10, 1980·Biochimica Et Biophysica Acta·P R Rich, D S Bendall

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Citations

Dec 1, 1992·Photosynthesis Research·J C Gray
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·A KatohT Ogawa
Jan 18, 1993·FEBS Letters·Y SasakiR Matsuno

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