An optimized and validated (1)H NMR method for the quantification of α-pinene in essentials oils

Talanta
Cristiane I CerceauPaulo H Fidencio

Abstract

The authenticity and composition of commercial essential oils requires strict quality control. Due to the importance of α-pinene containing essential oils, a rapid and efficient method for quantification of this terpene in oils of eucalyptus, pink pepper and turpentine using (1)H NMR was developed and validated. All evaluated parameters (selectivity, linearity, accuracy/precision, repeatability, robustness, stability of analyte and internal standard in solutions) showed satisfactory results. The limit of detection (LOD) and limit of quantification (LOQ) were 0.1 and 2.5mg respectively. These values indicated that α-pinene was detected in 35 mg samples containing at least 0.3% of this compound. In addition, a minimum of 8% of α-pinene in the sample was required for quantification. Furthermore, the standard deviations found in the (1)H NMR methodology were less than 1% and were lower than those obtained by gas chromatographic analysis. Statistical tests have shown that the results obtained by (1)H NMR methodology are similar to those obtained by GC-FID technique using external and internal standardization and normalization within 95% confidence. R&R values lower than 10% have shown that all the methods are appropriate and the (1)...Continue Reading

References

Feb 15, 2001·Journal of Agricultural and Food Chemistry·I J Kosir, J Kidric
May 17, 2005·Journal of Pharmaceutical and Biomedical Analysis·F Malz, H Jancke
Sep 17, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Luiz Cláudio Almeida BarbosaEvandro de Castro Melo
May 15, 2007·Talanta·Iñaki BerregiJosé Ignacio Miranda
Jan 1, 2009·International Journal of Occupational Medicine and Environmental Health·Beatrice MercierMichel Prost
Jul 1, 2010·Magnetic Resonance in Chemistry : MRC·Andersson BarisonAntonio Gilberto Ferreira
Jan 29, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Antonio Jacinto DemunerAntonio Lelis Pinheiro
Apr 10, 2012·Journal of Pharmaceutical and Biomedical Analysis·Ming-juan WangErwin Adams

❮ Previous
Next ❯

Citations

Apr 23, 2016·Chemistry & Biodiversity·Anne-Marie NamAnge Bighelli
Feb 27, 2019·Chemistry & Biodiversity·Nemesio Villa-RuanoElvia Becerra-Martínez
Jul 31, 2019·Phytochemical Analysis : PCA·Cristiane I CerceauFyaz M D Ismail
Jan 8, 2020·Phytochemical Analysis : PCA·Elvia Becerra-MartínezNemesio Villa-Ruano
Dec 10, 2020·Journal of Pharmaceutical and Biomedical Analysis·Faegheh FarhadiMehrdad Iranshahi
Apr 6, 2020·Food Research International·Rodolfo Velásquez-ValleElvia Becerra-Martínez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.