journal cover

An optimized CRISPR/Cas toolbox for efficient germline and somatic genome engineering in Drosophila

bioRxiv

Mar 24, 2014

Fillip PortSimon L Bullock

Abstract

The type II CRISPR/Cas system has recently emerged as a powerful method to manipulate the genomes of various organisms. Here, we report a novel toolbox for high efficiency genome engineering of Drosophila melanogaster consisting of transgenic Cas9 lines and versatile guide RNA (gRNA) ex...read more

Mentioned in this Paper

Drosophila
Evaluation
Disease Transmission
Nucleotides
Oligonucleotides
Transgenes
Plasmids
Recombination, Genetic
U6 small nuclear RNA
Base Sequence
46
34
Paper Details
References
  • References
  • Citations
  • finger pointing at paper

    References currently unavailable

    We're still populating references for this paper, please check back later.
  • References
  • Citations
  • quote and clock

    No citations available

    This paper may not have been cited yet.

Similar Papers Found In These Feeds

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

BioRxiv Preprints

BioRxiv is the preprint server for biology, operated by Cold Spring Harbor Laboratory. Here are the latest biology preprint articles from bioRxiv.

CRISPR Genome Editing & Therapy

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of this system for gene editing and therapy in human diseases.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

DNA Repair & Genome Editing

Development of gene editing techniques, including CRISPR and TALENs, has relied on mechanisms that underlie DNA repair and genome stability pathways. This feed focuses on the role of DNA repair mechanisms in gene editing and genetic engineering.

CRISPR for Genome Editing (Preprints)

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here are the latest preprints on the use of CRISPR-Cas system in gene editing.

CRISPR Genome Editing & Therapy (Preprints)

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of this system for gene editing and therapy in human diseases.

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

© 2020 Meta ULC. All rights reserved

An optimized CRISPR/Cas toolbox for efficient germline and somatic genome engineering in Drosophila

bioRxiv

Mar 24, 2014

Fillip PortSimon L Bullock

PMID: 990003541

DOI: 10.1101/003541

Abstract

The type II CRISPR/Cas system has recently emerged as a powerful method to manipulate the genomes of various organisms. Here, we report a novel toolbox for high efficiency genome engineering of Drosophila melanogaster consisting of transgenic Cas9 lines and versatile guide RNA (gRNA) ex...read more

Mentioned in this Paper

Drosophila
Evaluation
Disease Transmission
Nucleotides
Oligonucleotides
Transgenes
Plasmids
Recombination, Genetic
U6 small nuclear RNA
Base Sequence
46
34

Similar Papers Found In These Feeds

Related Papers

Paper Details
References
  • References
  • Citations
  • finger pointing at paper

    References currently unavailable

    We're still populating references for this paper, please check back later.
  • References
  • Citations
  • quote and clock

    No citations available

    This paper may not have been cited yet.
/papers/an-optimized-crisprcas-toolbox-for-efficient/990003541