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An optimized CRISPR/Cas toolbox for efficient germline and somatic genome engineering in Drosophila

bioRxiv

Mar 24, 2014

Fillip PortSimon L. Bullock

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Abstract

The type II CRISPR/Cas system has recently emerged as a powerful method to manipulate the genomes of various organisms. Here, we report a novel toolbox for high efficiency genome engineering of Drosophila melanogaster consisting of transgenic Cas9 lines and versatile guide RNA (gRNA) ex...read more

Mentioned in this Paper

CRISPR-Cas Systems
Type 2
Mutant
Drosophila melanogaster
Evaluation
Homologous Recombination Pathway
Oligonucleotides
Mutagenesis Process
Genome
Diploid Cell
46
34
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  • An optimized CRISPR/Cas toolbox for efficient germline and somatic genome engineering in Drosophila

    bioRxiv

    Mar 24, 2014

    Fillip PortSimon L. Bullock

    PMID: 990003541

    DOI: 10.1101/003541

    Abstract

    The type II CRISPR/Cas system has recently emerged as a powerful method to manipulate the genomes of various organisms. Here, we report a novel toolbox for high efficiency genome engineering of Drosophila melanogaster consisting of transgenic Cas9 lines and versatile guide RNA (gRNA) ex...read more

    Mentioned in this Paper

    CRISPR-Cas Systems
    Type 2
    Mutant
    Drosophila melanogaster
    Evaluation
    46
    34

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