An optimized culture medium for human vascular endothelial cells from umbilical cord veins.

Cytotechnology
P FriedlR Czpla

Abstract

Various polypeptide growth factors, culture substrates, basal media, sera and further supplements were assayed for improvement of growth of human vascular endothelial cells from umbilical cord veins. The resulting optimized medium consisted of gelatinized culture substrates, a mixture (1:1) of Iscove's MDM and Ham's F12 basal media supplemented with 20% newborn calf serum, 500 ng/ml crude fibroblast growth factor, 20 ng/ml epidermal growth factor, 5 μg/ml transferrin, 5 μg/ml insulin and 10 μg/ml heparin. The medium allowed long term cultivation of HUVEC up to 45 generations with maximal cell densities of about 10(5) cells per cm(2) and a minimal doubling time of about 14 hours at low cell densities.

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Citations

Jan 1, 1994·Cytotechnology·G SchrimpfP Friedl
Nov 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K Engelmann, P Friedl
Mar 10, 2004·Journal of Biotechnology·Martin SchröderPeter Friedl
May 18, 2000·Biochemical and Biophysical Research Communications·M A FreybergP Friedl
Aug 4, 2001·Biochemical and Biophysical Research Communications·M A FreybergP Friedl
Apr 1, 1990·International Journal of Biological Macromolecules·K R JergP Friedl
Jan 1, 1997·Endothelium : Journal of Endothelial Cell Research·M TeifelP Friedl
Sep 4, 1999·Endothelium : Journal of Endothelial Cell Research·D KaiserP Friedl
Feb 24, 1997·Biochemical and Biophysical Research Communications·D KaiserP Friedl
Nov 4, 2015·PloS One·Petra KoudelkovaWolfgang Mikulits
Jul 27, 2017·Cardiovascular Engineering and Technology·Marco Franzoni, Michael T Walsh
Mar 22, 2020·Biomaterials·Jefferson FrigugliettiFatima A Merchant
Sep 3, 2002·Microvascular Research·K PetersJ Rauterberg

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