Abstract
Quantifying the ability of a compound to modulate cell migration rate is a crucial part of many studies including those on chemotaxis, wound healing and cancer metastasis. Existing migration assays all have their strengths and weaknesses. The "scratch" assay is the most widely used because it seems appealingly simple and inexpensive. However, the scratch assay has some important limitations, as the tool introducing the "wound" might injure/stress the boundary cells and/or harm underlying matrix coatings, which in both cases will affect cell migration. This described method is a Cell Exclusion Zone Assay, in which cell-free areas are created by growing cells around removable silicone stoppers. Upon appropriate staining with fluorescent dyes and microscopically visualizing the monolayers, the migration rate is then quantified by counting the cells (nuclei) intruding the void area left by the silicone insert. In the current study human small intestine epithelial cells were seeded on a physiological substrate matrix to produce collectively migrating monolayers. Different substrates were tested to determine the optimal surface for enterocyte adherence and migration and morphological changes monitored. Recombinant human epidermal gro...Continue Reading
Citations
Jan 27, 2018·Bulletin of Mathematical Biology·Oleksii M MatsiakaMatthew J Simpson
Dec 19, 2017·Frontiers in Cellular and Infection Microbiology·Jungnam LeeÖzlem Yilmaz
Jul 3, 2018·GigaScience·Marcela M Fernandez-GutierrezAndrew P Stubbs
May 5, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Fang WangChangcheng Zhou
Sep 4, 2019·Scientific Reports·M FenuI Ribitsch
Mar 7, 2020·Glia·Bilge DundarDaniel J Brat
Apr 3, 2020·Journal of Cellular Physiology·Gastón AmableOsvaldo Rey
Jun 26, 2018·Oncotarget·Carolina GarriKian Kani
Jun 6, 2020·Plants·Pornpatsorn LertphadungkitSomnuk Bunsupa
Jul 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Francis Yew Fu TiengAgustono Wibowo
Jul 28, 2017·Food Science & Nutrition·Steffen NyegaardJan Trige Rasmussen
Jul 30, 2020·PloS One·Alejandra Suarez-ArnedoCarolina Muñoz-Camargo
Jun 16, 2019·Bulletin of Mathematical Biology·Ana Victoria Ponce BobadillaTomás Alarcón
Mar 17, 2020·BMC Cancer·Jinzhi LiGang Liu
Nov 26, 2020·Biofabrication·Raymond TranChristopher Moraes
Aug 10, 2020·Cancer Letters·Shao Thing TeohSophia Y Lunt
Jul 17, 2021·Journal of Neuroscience Methods·Pascal AchenbachGary A Brook
Jan 1, 2019·ACS Biomaterials Science & Engineering·Catalina-Paula SpatareluZi Chen
Sep 2, 2021·PloS One·Mallory J DiVincenzoWilliam E Carson