An optimized method for accurate quantification of cell migration using human small intestine cells

Metabolic Engineering Communications
Steffen NyegaardJan Trige Rasmussen

Abstract

Quantifying the ability of a compound to modulate cell migration rate is a crucial part of many studies including those on chemotaxis, wound healing and cancer metastasis. Existing migration assays all have their strengths and weaknesses. The "scratch" assay is the most widely used because it seems appealingly simple and inexpensive. However, the scratch assay has some important limitations, as the tool introducing the "wound" might injure/stress the boundary cells and/or harm underlying matrix coatings, which in both cases will affect cell migration. This described method is a Cell Exclusion Zone Assay, in which cell-free areas are created by growing cells around removable silicone stoppers. Upon appropriate staining with fluorescent dyes and microscopically visualizing the monolayers, the migration rate is then quantified by counting the cells (nuclei) intruding the void area left by the silicone insert. In the current study human small intestine epithelial cells were seeded on a physiological substrate matrix to produce collectively migrating monolayers. Different substrates were tested to determine the optimal surface for enterocyte adherence and migration and morphological changes monitored. Recombinant human epidermal gro...Continue Reading

Citations

Jan 27, 2018·Bulletin of Mathematical Biology·Oleksii M MatsiakaMatthew J Simpson
Jul 3, 2018·GigaScience·Marcela M Fernandez-GutierrezAndrew P Stubbs
May 5, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Fang WangChangcheng Zhou
Jul 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Francis Yew Fu TiengAgustono Wibowo
Jul 28, 2017·Food Science & Nutrition·Steffen NyegaardJan Trige Rasmussen
Jul 30, 2020·PloS One·Alejandra Suarez-ArnedoCarolina Muñoz-Camargo
Jun 16, 2019·Bulletin of Mathematical Biology·Ana Victoria Ponce BobadillaTomás Alarcón
Nov 26, 2020·Biofabrication·Raymond TranChristopher Moraes
Jan 1, 2019·ACS Biomaterials Science & Engineering·Catalina-Paula SpatareluZi Chen
Sep 2, 2021·PloS One·Mallory J DiVincenzoWilliam E Carson

❮ Previous
Next ❯

Methods Mentioned

BETA
FRET
scraping

Software Mentioned

CellMask
ImageJ
Image→Adjust→Threshold
Graphpad Prism
Olympus Cell ^ B
Analyze→Analyze
Excel

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.