An optimized model for rat liver perfusion studies

The Journal of Surgical Research
K CheungM S Roberts

Abstract

Conditions which influence the viability, integrity, and extraction efficiency of the isolated perfused rat liver were examined to establish optimal conditions for subsequent work in reperfusion injury studies including the choice of buffer, use of oncotic agents, hematocrit, perfusion flow rate, and pressure. Rat livers were perfused with MOPS-buffered Ringer solution with or without erythrocytes. Perfusates were collected and analyzed for blood gases, electrolytes, enzymes, radioactivity in MID studies, and lignocaine in extraction studies. Liver tissue was sampled for histological examinations, and wet:dry weight of the liver was also determined. MOPS-buffered Ringer solution was found to be superior to Krebs bicarbonate buffer, in terms of pH control and buffering capacity, especially during any prolonged period of liver perfusion. A pH of 7. 2 is chosen for perfusion since this is the physiological pH of the portal blood. The presence of albumin was important as an oncotic agent, particularly when erythrocytes were used in the perfusate. Perfusion pressure, resistance, and vascular volume are flow-dependent and the inclusion of erythrocytes in the perfusate substantially altered the flow characteristics for perfusion press...Continue Reading

Citations

Jul 25, 2000·British Journal of Pharmacology·M WeissM S Roberts
Nov 14, 2014·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Hossein-Ali ArabMichael S Roberts
Aug 4, 1998·Journal of Pharmaceutical Sciences·D Y HungM S Roberts
Oct 20, 2012·Journal of Pharmacological and Toxicological Methods·Andrea FerrignoMariapia Vairetti
Feb 16, 2005·British Journal of Pharmacology·Daniel Y HungMichael S Roberts
Jul 15, 2011·Biotechnology and Bioengineering·Mehmet A OrmanFrancois Berthiaume
Mar 12, 2004·Chemico-biological Interactions·James L MaggsB Kevin Park
Jun 26, 2001·The Journal of Surgical Research·S Rodríguez-ReynosoC Leal
Sep 30, 2010·Drug Metabolism and Pharmacokinetics·Yu-Hong ZouMichael S Roberts
Jul 24, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Peng LiMichael S Roberts
May 11, 2005·The Journal of Surgical Research·Scott BantaMartin L Yarmush
Jan 26, 2007·Pharmaceutical Research·Kent John Fanning, Michael S Roberts
Oct 20, 2006·Journal of Pharmacokinetics and Pharmacodynamics·C Anthony HuntMichael S Roberts
Oct 24, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Daniel Y HungMichael S Roberts
Apr 23, 2005·Digestive Diseases and Sciences·Ping ChangMichael S Roberts
Mar 19, 2008·Biopharmaceutics & Drug Disposition·Mohammad-Reza RouiniHoda Lavasani
Feb 28, 2007·Clinical and Experimental Pharmacology & Physiology·H A ArabM S Roberts
Sep 17, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Daniel Y HungMichael S Roberts
Jan 20, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Peng LiMichael S Roberts
Oct 21, 2003·The Journal of Pharmacology and Experimental Therapeutics·Gerhard A SiebertMichael S Roberts
Jun 12, 2004·The Journal of Pharmacology and Experimental Therapeutics·Daniel Y HungMichael S Roberts
Oct 25, 2008·The Journal of Pharmacology and Experimental Therapeutics·Sunwoo ParkC Anthony Hunt
May 23, 2002·The Journal of Pharmacology and Experimental Therapeutics·Daniel Y HungMichael S Roberts
May 8, 1999·The American Journal of Physiology·M G LatourJ M Lavoie
Nov 22, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Daniel Y HungMichael S Roberts
Oct 30, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Michael S RobertsDarrell H G Crawford
Sep 4, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Daniel Y HungMichael S Roberts
Mar 29, 2005·Molecular and Cellular Biochemistry·F J BurczynskiS Robert
Apr 29, 2009·Journal of Agricultural and Food Chemistry·Siyuan ZhouQibing Mei

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