journal cover

An optimized protocol for generation and analysis of Ion Proton sequencing reads for RNA-Seq

BMC Genomics

May 28, 2016

Yongxian YuanRoss Ka-Kit Leung

Abstract

Previous studies compared running cost, time and other performance measures of popular sequencing platforms. However, comprehensive assessment of library construction and analysis protocols for Proton sequencing platform remains unexplored. Unlike Illumina sequencing platforms, Proton r...read more

Mentioned in this Paper

Computer Software
Study
Proton Therapy
Abnormal Fragmented Structure
Electrophoresis, Agar Gel
Exons
Genome
Genes
Sequence Determinations, RNA
Workflow
11
57
Paper Details
References
  • References55
  • Citations6
12345...

Feeds With Similar Papers

RNA Splicing (Keystone)

The majority of human genes that encode proteins undergo alternative pre-mRNA splicing and mutations that affect splicing are more prevalent than previously thought. Discover the latest research on RNA splicing and co-transcriptional splicing in this feed.

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

Exon Skipping

Exon skipping is a method of RNA splicing used when a mutation occurs in the genes of exons. It is regulated largely by the use of antisense oligonucleotides that mask the mutated gene and allow the cellular machinery to skip over it. Exon skipping is being investigated as a therapeutic option for some diseases. Here is the latest research on exon skipping.

Structural Biology: Biophysics

Structural Biology: Biophysics describes the study of structural biology using various biophysical techiniques such as imaging, computational modeling, etc. Discover the latest research on structrual biology here.

RNA Splicing

The majority of human genes that encode proteins undergo alternative pre-mRNA splicing and mutations that affect splicing are more prevalent than previously thought. Discover the latest research on RNA Splicing here.

Perturb-seq

Perturb-seq has combined CRISPR gene inactivation and single-cell rna-sequencing (scRNA-seq) and is the newest addition to the geneticist's arsenal, providing scientists with study methods for functional genomics. Discover the latest research here.

Genome Annotation

Genome annotation is the process of identifying genes and assigning function to each gene in a genome sequence. It provides the means to elucidate biological function from sequence. Here is the latest research.

Related Papers

Bioinformatics

OSA: a fast and accurate alignment tool for RNA-Seq

BioinformaticsMay 18, 2012
Jun HuKejun Liu
PloS One

A comprehensive evaluation of alignment algorithms in the context of RNA-seq

PloS OneJanuary 10, 2013
Robert Lindner, Caroline C Friedel
© 2020 Meta ULC. All rights reserved

An optimized protocol for generation and analysis of Ion Proton sequencing reads for RNA-Seq

BMC Genomics

May 28, 2016

Yongxian YuanRoss Ka-Kit Leung

PMID: 27229683

DOI: 10.1186/s12864-016-2745-8

Abstract

Previous studies compared running cost, time and other performance measures of popular sequencing platforms. However, comprehensive assessment of library construction and analysis protocols for Proton sequencing platform remains unexplored. Unlike Illumina sequencing platforms, Proton r...read more

Mentioned in this Paper

Computer Software
Study
Proton Therapy
Abnormal Fragmented Structure
Electrophoresis, Agar Gel
Exons
Genome
Genes
Sequence Determinations, RNA
Workflow
11
57

Feeds With Similar Papers

RNA Splicing (Keystone)

The majority of human genes that encode proteins undergo alternative pre-mRNA splicing and mutations that affect splicing are more prevalent than previously thought. Discover the latest research on RNA splicing and co-transcriptional splicing in this feed.

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

Related Papers

Bioinformatics

OSA: a fast and accurate alignment tool for RNA-Seq

BioinformaticsMay 18, 2012
Jun HuKejun Liu
PloS One

A comprehensive evaluation of alignment algorithms in the context of RNA-seq

PloS OneJanuary 10, 2013
Robert Lindner, Caroline C Friedel
Paper Details
References
  • References55
  • Citations6
12345...

Download from

Publisher
PubMed
/papers/an-optimized-protocol-for-generation-and-analysis/27229683