An optimized surgical approach for obtaining stable extracellular single-unit recordings from the cerebellum of head-fixed behaving mice

Journal of Neuroscience Methods
Joshua J WhiteRoy V Sillitoe

Abstract

Electrophysiological recording approaches are essential for understanding brain function. Among these approaches are various methods of performing single-unit recordings. However, a major hurdle to overcome when recording single units in vivo is stability. Poor stability results in a low signal-to-noise ratio, which makes it challenging to isolate neuronal signals. Proper isolation is needed for differentiating a signal from neighboring cells or the noise inherent to electrophysiology. Insufficient isolation makes it impossible to analyze full action potential waveforms. A common source of instability is an inadequate surgery. Problems during surgery cause blood loss, tissue damage and poor healing of the surrounding tissue, limited access to the target brain region, and, importantly, unreliable fixation points for holding the mouse's head. We describe an optimized surgical procedure that ensures limited tissue damage and delineate a method for implanting head plates to hold the animal firmly in place. Using the cerebellum as a model, we implement an extracellular recording technique to acquire single units from Purkinje cells and cerebellar nuclear neurons in behaving mice. We validate the stability of our method by holding si...Continue Reading

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Citations

Apr 5, 2017·Nature Communications·Joshua J White, Roy V Sillitoe
Feb 12, 2019·Scientific Reports·Amanda M BrownRoy V Sillitoe
Apr 12, 2017·Current Protocols in Neuroscience·Sabrina L LevyRoy V Sillitoe
Aug 1, 2018·Frontiers in Cell and Developmental Biology·Thomas W P Wood, Tetsuya Nakamura
Nov 11, 2019·Disease Models & Mechanisms·Trace L StayRoy V Sillitoe
May 28, 2020·Frontiers in Bioengineering and Biotechnology·Shreya MahajanEvon S Ereifej
Dec 29, 2020·The Journal of Physiology·Meike E van der HeijdenRoy V Sillitoe

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