An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase

Chemistry : a European Journal
Ping WangLing Yang

Abstract

A practical two-photon fluorescent probe was developed for highly sensitive and selective sensing of the activities of catechol-O-methyltransferase (COMT) in complex biological samples. To this end, a series of 3-substituted 7,8-dihydroxycoumarins were designed and synthesized. Among them, 3-BTD displayed the best combination of selectivity, sensitivity, reactivity, and fluorescence response following COMT-catalyzed 8-O-methylation. The newly developed two-photon fluorescent probe 3-BTD can be used for determining the activities of COMT in complex biological samples and bio-imaging of endogenous COMT in living cells and tissue slices with good cell permeability, low cytotoxicity, and high imaging resolution. All these findings suggest that 3-BTD holds great promise for developing therapeutic molecules that target COMT, as well as for exploring COMT-associated biological processes and its biological functions in living systems. Furthermore, the strategy also sheds new light on the development of fluorescent probes for other conjugative enzymes.

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Citations

Aug 25, 2018·Chemical Society Reviews·Hong-Wen LiuWeihong Tan
Sep 29, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yangliu XiaPing Wang
Aug 17, 2019·Expert Opinion on Drug Discovery·Rafael Ferreira DantasFloriano Paes Silva-Junior
Mar 16, 2021·Journal of Pharmaceutical Analysis·Fang-Yuan WangLing Yang
May 26, 2021·Journal of Enzyme Inhibition and Medicinal Chemistry·Fang-Yuan WangLing Yang

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