An optomechanical transducer in the blue light receptor phototropin from Avena sativa

Proceedings of the National Academy of Sciences of the United States of America
M SalomonG Richter

Abstract

The PHOT1 (NPH1) gene from Avena sativa specifies the blue light receptor for phototropism, phototropin, which comprises two FMN-binding LOV domains and a serine/threonine protein kinase domain. Light exposure is conducive to autophosphorylation of the protein kinase domain. We have reconstituted a recombinant LOV2 domain of A. sativa phototropin with various (13)C/(15)N-labeled isotopomers of the cofactor, FMN. The reconstituted protein samples were analyzed by NMR spectroscopy under dark and light conditions. Blue light irradiation is shown to result in the addition of a thiol group (cysteine 450) to the 4a position of the FMN chromophore. The adduct reverts spontaneously in the dark by elimination. The light-driven flavin adduct formation results in conformational modification, which was diagnosed by (1)H and (31)P NMR spectroscopy. This conformational change is proposed to initiate the transmission of the light signal via conformational modulation of the protein kinase domain conducive to autophosphorylation of NPH1.

References

Aug 15, 1985·European Journal of Biochemistry·J VervoortH Sedlmaier
Mar 1, 1996·Plant Physiology
Sep 25, 1997·Trends in Biochemical Sciences·I B ZhulinR Dixon
Jun 5, 1999·Microbiology and Molecular Biology Reviews : MMBR·B L Taylor, I B Zhulin
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·J M ChristieW R Briggs
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·S Crosson, K Moffat

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Citations

Apr 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Shin-Ichiro InoueKen-Ichiro Shimazaki
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kaiyao Huang, Christoph F Beck
Nov 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Erin B PurcellSean Crosson
Mar 3, 2010·Annual Review of Plant Biology·Andreas MöglichKeith Moffat
Apr 20, 2007·European Biophysics Journal : EBJ·Valentina ButtaniAba Losi
Feb 27, 2009·Antioxidants & Redox Signaling·Toshiya SendaTetsuo Ishida
Oct 18, 2008·Science·Jeeyeon LeeRama Ranganathan
Jan 16, 2003·Biochimica Et Biophysica Acta·Marco H HeftiWillem J H van Berkel
Oct 18, 2002·The Plant Journal : for Cell and Molecular Biology·John M ChristieWinslow R Briggs
Feb 20, 2016·Chemical Reviews·Nikolay V Dokholyan
Sep 25, 2015·Protein Science : a Publication of the Protein Society·Rang LeeJeeyeon Lee
Sep 21, 2010·Current Opinion in Neurobiology·Anshul Rana, Ricardo E Dolmetsch
Jun 29, 2010·Journal of Plant Physiology·Min OuyangLixin Zhang
May 18, 2010·Journal of Molecular Biology·Andreas MöglichKeith Moffat
Sep 17, 2008·Journal of Molecular Biology·Yusuke NakasoneMasahide Terazima
Nov 9, 2007·Biochimica Et Biophysica Acta·Satoru TokutomiKazunori Zikihara
Oct 8, 2015·Annual Review of Genetics·Aretha FiebigSean Crosson
Sep 4, 2007·Journal of Molecular Biology·Andreas Möglich, Keith Moffat
Jul 10, 2007·Journal of Molecular Biology·Takeshi EitokuMasahide Terazima
Aug 6, 2015·The Journal of Physical Chemistry. B·Wolfgang EisenreichStefan Weber
Nov 19, 2003·European Journal of Biochemistry·Marco H HeftiWillem J H van Berkel
Jun 11, 2009·Photochemistry and Photobiology·Anne-Kathrin HendrischkGabriele Klug
Jun 29, 2006·Photochemistry and Photobiology·Daisuke MatsuokaSatoru Tokutomi
Oct 20, 2007·The FEBS Journal·Wolfgang EisenreichMarkus Fischer
Nov 22, 2007·Photochemistry and Photobiology·Aba Losi
Jan 5, 2011·Photochemistry and Photobiology·Stefan WeberRobert Bittl
Oct 3, 2012·Photochemistry and Photobiology·Ruby H ChanRoberto A Bogomolni
Jan 26, 2011·Photochemistry and Photobiology·Ivo H M van StokkumJohn T M Kennis
Dec 22, 2006·IEEE Transactions on Nanobioscience·Sukhdev Roy, Kapil Kulshrestha
Sep 24, 2004·Journal of Molecular Biology·Markus FischerWolfgang Eisenreich
May 10, 2005·Biophysical Journal·Huimin GuoBernhard Dick
Sep 22, 2015·Frontiers in Molecular Biosciences·Daniel NohrErik Schleicher
Aug 29, 2009·Journal of Molecular Biology·Jessica S LambLois Pollack
Dec 1, 2004·Annual Review of Genetics·Meng ChenChristian Fankhauser
Oct 20, 2009·Structure·Andreas MöglichKeith Moffat
Dec 27, 2011·Cell·Kimberly A ReynoldsRama Ranganathan
Nov 26, 2008·Journal of Molecular Biology·Tilo MathesPeter Hegemann
Dec 27, 2008·Journal of Molecular Biology·Andreas MöglichKeith Moffat
Apr 20, 2002·Biophysical Journal·Aba LosiWolfgang Gärtner

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