An oral versus intranasal prime/boost regimen using attenuated human rotavirus or VP2 and VP6 virus-like particles with immunostimulating complexes influences protection and antibody-secreting cell responses to rotavirus in a neonatal gnotobiotic pig model.

Clinical and Vaccine Immunology : CVI
Marli P AzevedoLinda J Saif

Abstract

We determined the impact of mucosal prime/boost regimens and vaccine type (attenuated Wa human rotavirus [AttHRV] or nonreplicating Wa 2/6 rotavirus-like particles [VLP]) on protection and antibody-secreting cell (ASC) responses to HRV in a neonatal gnotobiotic pig disease model. Comparisons of delivery routes for AttHRV and evaluation of nonreplicating VLP vaccines are important as alternative vaccine approaches to overcome risks associated with live oral vaccines. Groups of neonatal gnotobiotic pigs were vaccinated using combinations of oral (PO) and intranasal (IN) inoculation routes as follows: (i) 3 oral doses of AttHRV (AttHRV3xPO); (ii) AttHRV3xIN; (iii) AttHRVPO, then 2/6VLP2xIN; (iv) AttHRVIN, then 2/6VLP2xIN; and (v) mock-inoculated controls. Subsets of pigs from each group were challenged with virulent Wa HRV [P1A(8) G1] (4 weeks post-primary inoculation) to assess protection. The AttHRVPO+2/6VLP2xIN pigs had the highest protection rates against virus shedding and diarrhea (71% each); however, these rates did not differ statistically among the vaccine groups, except for the AttHRVIN+2/6VLPIN group, which had a significantly lower protection rate (17%) against diarrhea. The isotype, magnitude, and tissue distribution ...Continue Reading

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Citations

Sep 2, 1998·Immunology and Cell Biology·F Morein, K L Bengtsson
Jul 25, 2012·Therapeutic Delivery·Thanigaivel Shanmugam, Rinti Banerjee
Oct 8, 2014·Therapeutic Delivery·Melissa M Herbst-Kralovetz
Aug 3, 2011·Expert Review of Vaccines·Jimmy A Mena, Amine A Kamen
Jun 10, 2011·Vaccine·Anton P J MiddelbergMichael R Batzloff
Aug 31, 2013·Veterinary Immunology and Immunopathology·Caroline FossumKarin Lövgren Bengtsson
Sep 23, 2014·Molecular Immunology·Heather L Wilson, Milan R Obradovic
Apr 4, 2013·Molecular Pharmaceutics·Gaëlle VacherGerrit Borchard
Jul 19, 2019·Clinical and Experimental Immunology·R R C New
Aug 27, 2014·Expert Review of Vaccines·Shih-Yeh LinYu-Chen Hu
Dec 31, 2020·Vaccines·Hans Van der WekenBert Devriendt
Feb 20, 2021·Human Vaccines & Immunotherapeutics·Volker VetterPriya Pereira

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