Abstract
A thermophile, halotolerant and organic-solvent-tolerant esterase producer Bacillus sp. S-86 strain previously isolated was found to belong to Bacillus licheniformis species through morphological, biochemical, 16S rRNA gene sequence analyses and rDNA intergenic spacers amplification (ITS-PCR). The strain can grow at 55 degrees C in presence of C2-C7 alkanols (log P=-0.86 to 2.39), and NaCl concentrations up to 15% (w/v). This bacterium showed optimal growth and esterase production at 50 degrees C. Two different molecular weight esterase activities were detected in zymographic assays. PMSF inhibited type I esterase activity, showing no inhibitory effect on type II esterase activity. B. licheniformis S-86 was able to grow in presence of hydroxylic organic-solvents like propan-2-ol, butan-1-ol and 3-methylbutan-1-ol. At a sub-lethal concentration of these solvents (392 mmoll(-1) propan-2-ol; 99 mmol l(-1) butan-1-ol, 37 mmol l(-1) 3-methylbutan-1-ol), adequate to produce 50% cell growth inhibition at 50 degrees C, an increment between 1.9 and 2.3 times was observed in type I esterase production, and between 2.2 and 3.1 times in type II esterase production.
References
May 1, 1977·Analytical Biochemistry·J J Sedmak, S E Grossberg
May 1, 1995·Applied and Environmental Microbiology·M M YakimovH L Fredrickson
Aug 10, 2000·FEMS Microbiology Letters·G R BrownS P Cummings
May 15, 2002·FEMS Microbiology Reviews·Uwe T Bornscheuer
Aug 6, 2002·Research in Microbiology·Yogita Sardessai, Saroj Bhosle
Sep 7, 2002·Extremophiles : Life Under Extreme Conditions·Mark T BustardPhillip C Wright
Oct 17, 2002·Extremophiles : Life Under Extreme Conditions·M Alejandra MartínezFaustino Siñeriz
Jun 17, 2003·International Journal of Systematic and Evolutionary Microbiology·Dong Xu, Jean-Charles Côté
Jul 24, 2003·Marine Biotechnology·Yogita Sardessai, Saroj Bhosle
Jun 23, 2004·European Journal of Biochemistry·Munishwar N Gupta, Ipsita Roy
Oct 20, 2004·Bioresource Technology·Raja Noor Zaliha Abd RahmanAbu Bakar Salleh
Mar 19, 2005·International Journal of Systematic and Evolutionary Microbiology·Virginia A VargasBo Mattiasson
Mar 19, 2005·International Journal of Systematic and Evolutionary Microbiology·Isao YumotoKenji Nakajima
Apr 5, 2005·Archaea : an International Microbiological Journal·Taku AmoTadayuki Imanaka
May 10, 2005·International Journal of Systematic and Evolutionary Microbiology·Cristina Ruiz-GarcíaVictoria Béjar
Sep 17, 2005·International Journal of Systematic and Evolutionary Microbiology·Jürgen HeyerPeter F Dunfield
Sep 17, 2005·International Journal of Systematic and Evolutionary Microbiology·Jun TsubotaYuri A Trotsenko
Oct 1, 1994·Applied and Environmental Microbiology·H OginoH Ishikawa
Mar 15, 2006·Extremophiles : Life Under Extreme Conditions·Jiunn C N FongPeter L Rogers
Oct 10, 2006·Applied and Environmental Microbiology·Jens WaldeckFriedhelm Meinhardt
Jan 31, 2007·Applied Microbiology and Biotechnology·Hermann J HeipieperFriedhelm Meinhardt
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jun 27, 2007·Bioresource Technology·Srivathsan Vembanur RanganathanKaruppan Muthukumar
Jul 13, 2007·International Journal of Systematic and Evolutionary Microbiology·Keiko WatanabeNorio Kurosawa
Oct 10, 2007·Current Opinion in Microbiology·Konstantinos T Konstantinidis, James M Tiedje
Mar 19, 2008·Bioresource Technology·X Y TangB F He
Citations
Nov 15, 2011·Applied Microbiology and Biotechnology·Tong-Guo GaoHong-Li Yuan
Dec 9, 2014·Jundishapur Journal of Microbiology·Saeed ZargariShahab Ayatollahi
Jan 15, 2016·Biological Procedures Online·Ashok KumarPankaj Kumar Arora
Mar 20, 2012·Bioresource Technology·Andrés IllanesGuillermo R Castro
Apr 21, 2011·Biotechnology Advances·Sebastian TorresGuillermo R Castro
Sep 15, 2010·Biotechnology Advances·Carla C C R de Carvalho
Dec 24, 2015·Applied Biochemistry and Biotechnology·Jian-Yong ZhengZhao Wang
Oct 16, 2015·Applied Biochemistry and Biotechnology·Jiayuan LiangYunfeng Hu
Nov 22, 2011·Bioresource Technology·Peng JinQiuyan Wang
Dec 31, 2014·Applied Microbiology and Biotechnology·Hongfei HuShaojun Ding
Apr 28, 2016·Applied Biochemistry and Biotechnology·Yingying CaoYunfeng Hu
Aug 20, 2014·PloS One·Meiyan YangGuanglei Zhao
Apr 21, 2018·Chirality·Jianyong ZhengZhao Wang
Jul 4, 2020·Scientific Reports·Jaya A GuptaKrishna Jyoti Mukherjee
Jul 20, 2020·European Biophysics Journal : EBJ·Ana Elisa T LeiteIgor Polikarpov
Feb 28, 2019·Applied Microbiology and Biotechnology·Mingji LuRolf Daniel
Mar 17, 2020·Carbohydrate Research·Zabin K BagewadiSikandar I Mulla
Feb 21, 2021·International Journal of Biological Macromolecules·Mayra Alejandra López-OrtegaAdriana Inés Rodríguez-Hernández
Jul 24, 2021·International Journal of Biological Macromolecules·Abdallah R IsmailKwang-Hyun Baek
Oct 26, 2010·Carbohydrate Research·Shailesh R Waghmare, Jai S Ghosh