An outbreak of classical swine fever in pigs in Bangladesh, 2015

Veterinary Medicine and Science
Shamim SarkarMohammed Z Rahman

Abstract

In a group of 22 healthy pigs aged between 4 and 6 months, 2 pigs became ill with high fever, complete anorexia, cough and abnormal swaying movements on 22 June 2015. One of them died on June 24 and the second died on July 3. Shortly after, the remaining pigs also fell ill and died from the same illness by 10 August 2015. We investigated the aetiology, epidemiological and clinical features of the outbreak. We recorded the clinical signs and symptoms for each pig with the date of onset of illness. Veterinarians conductedpost-mortemexaminations on the 12 dead pigs, they collected tissue samples from the dead pigs and placed them in a tube containing 1 mL of nucleic acid extraction buffer (lysis buffer). We tested all the tissue samples by real-time reverse transcription polymerase chain reaction (rRT-PCR) to detect classical swine fever virus (CSFV) because the animals' symptoms matched those of this disease. We also conducted a phylogentic analysis of the nucleotide sequence of the E2 gene segment of CSFV detected in a lung tissue sample. The attack rate (22/22) and the case fatality were 100%. The predominant symptoms of the disease included high fever, cough, diarrhoea and swaying movements of the hind legs prior to death. Of ...Continue Reading

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Citations

May 5, 2018·Tropical Animal Health and Production·Vinod Kumar SinghSharad Kumar Yadav

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Datasets Mentioned

BETA
KX345847

Methods Mentioned

BETA
nucleic acid extraction
genotyping
PCR

Software Mentioned

Molecular Evolutionary Genetics Analysis ( MEGA )

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