An outbreak of the polyomavirus infection in budgerigars and cockatiels in Slovakia, including a genome analysis of an avian polyomavirus isolate

Avian Diseases
Ivan LiterákLubomír Valícek

Abstract

In winter 2003-04, large numbers of budgerigars (Mellopsitacus undulatus) and cockatiels (Nymphicus hollandicus) fell ill and died in a large parrot-breeding aviary in Slovakia. In budgerigars, the disease outbreak occurred at the age of 2-3 weeks; cockatiels died within their first 7 days of life. In budgerigars, symptoms of the disease included delayed growth, tremor, darkish discoloration of skin, quill bleeding, and feathering defects. cockatiels often died without any symptoms and with a full crop; feathering defects occurred sporadically. Electron microscopy with negative staining of aqueous lysates of the affected skin and of bleeding quills showed isolated or clustered polyomavirus particles 45-50 nm in size. Long filamentous forms of the virus were also found in virion clusters of skin lysates from the budgerigars. In ultrathin sections through the pathologically altered skin tissue of budgerigars, virus particles were present in both nuclei and cytoplasm of epidermal cells, often in crystalline form. In infected cells, enlarged nuclei showed an extensive chromatin margination. On the DNA level, presence of a polyomavirus infection was conclusively proved by the polymerase chain reaction using avian polyomavirus (APV)-...Continue Reading

References

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Citations

Aug 24, 2007·Journal of Virology·Reimar Johne, Hermann Müller
Apr 29, 2010·The Journal of Veterinary Medical Science·Hiroshi KatohHideto Fukushi
Jun 19, 2013·Journal of Avian Medicine and Surgery·Brenna FitzgeraldBrian Speer
Jul 17, 2008·Biology of the Cell·Philippe Roingeard
Nov 10, 2018·Reviews in Medical Virology·Philippe RoingeardEmmanuelle Blanchard
Jun 30, 2019·Journal of Avian Medicine and Surgery·Gisela González-HeinBernardo Huaracan

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Datasets Mentioned

BETA
DQ074760
AF056497

Methods Mentioned

BETA
electrophoresis
PCR
electron
electron microscopy

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