An Outpatient Clinic as a Potential Site of Transmission for an Outbreak of New Delhi Metallo-β-Lactamase-producing Klebsiella pneumoniae Sequence Type 716: A Study Using Whole-genome Sequencing

Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
Amélie HeinrichsOlivier Denis

Abstract

The incidence of nosocomial infections due to carbapenem-resistant Klebsiella pneumoniae is increasing worldwide. Whole-genome sequencing (WGS) can help elucidate the transmission route of nosocomial pathogens. We combined WGS and epidemiological data to analyze an outbreak of New Delhi metallo-β-lactamase (NDM)-producing K. pneumoniae that occurred in 2 Belgian hospitals situated about 50 miles apart. We characterized 74 NDM-producing K. pneumoniae isolates (9 from hospital A, 24 from hospital B, and 41 contemporary isolates from 15 other Belgian hospitals) using pulsed-field gel electrophoresis and WGS. A K. pneumoniae sequence type 716 clone was identified as being responsible for the outbreak with all 9 strains from hospital A and 20 of 24 from hospital B sharing a unique pulsotype and being clustered together at WGS (compared with 1 of 41 isolates from other Belgian hospitals). We identified the outpatient clinic of hospital B as the probable bridging site between the hospitals after combining epidemiological, phylogenetic, and resistome data. We also identified the patient who probably caused the transmission. In fact, all but 1 strain from hospital A carried a Tn1331-like transposon, whereas none of the hospital B isolat...Continue Reading

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Citations

Feb 1, 2019·Clinical Microbiology Reviews·Wenjing WuZhiyong Zong
Feb 28, 2020·Clinical Microbiology Reviews·Karen Bush, Patricia A Bradford
Sep 29, 2020·Frontiers in Microbiology·Nahla O EltaiSurbhi Malhotra-Kumar
Sep 26, 2019·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Janko van BeekOuti Lyytikäinen
Nov 20, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sabrina KleinDennis Nurjadi
May 4, 2020·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·N Hassoun-KheirM Paul
Jun 24, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Maria A ArgudínMarie Hallin

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