An Overview of Peripheral Blood Mononuclear Cells as a Model for Immunological Research of Toxoplasma gondii and Other Apicomplexan Parasites

Frontiers in Cellular and Infection Microbiology
John Alejandro Acosta Davila, Alejandro Hernandez De Los Rios

Abstract

In biology, models are experimental systems meant to recreate aspects of diseases or human tissue with the goal of generating inferences and approximations that can contribute to the resolution of specific biological problems. Although there are many models for studying intracellular parasites, their data have produced critical contradictions, especially in immunological assays. Peripheral blood mononuclear cells (PBMCs) represent an attractive tissue source in pharmacogenomics and in molecular and immunologic studies, as these cells are easily collected from patients and can serve as sentinel tissue for monitoring physiological perturbations due to disease. However, these cells are a very sensitive model due to variables such as temperature, type of stimulus and time of collection as part of posterior processes. PBMCs have been used to study Toxoplasma gondii and other apicomplexan parasites. For instance, this model is frequently used in new therapies or vaccines that use peptides or recombinant proteins derived from the parasite. The immune response to T. gondii is highly variable, so it may be necessary to refine this cellular model. This mini review highlights the major approaches in which PBMCs are used as a model of stud...Continue Reading

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Citations

Oct 1, 2020·International Reviews of Immunology·Sahar Rostami MansoorHanie Yavarpour-Bali
Dec 24, 2019·Frontiers in Cellular and Infection Microbiology·Alejandro Hernández-de-Los-RíosJorge Enrique Gómez-Marín
Dec 11, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·Bernardo BonilauriJuliana de S da G Fischer
Apr 15, 2021·Transactions of the Royal Society of Tropical Medicine and Hygiene·Khadijeh KhanalihaBorna Salemi
Jul 31, 2021·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Fei PengConor L Evans
Oct 16, 2020·Experimental Parasitology·Alejandro Acosta-DávilaJorge Enrique Gómez-Marín
Jan 28, 2022·Clinical Chemistry and Laboratory Medicine : CCLM·Tanja GolkeHelmuth Haslacher

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Methods Mentioned

BETA
density-gradient centrifugation
RNAseq

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