An Overview of Tyrosine Site-specific Recombination: From an Flp Perspective

Microbiology Spectrum
M JayaramYuri Voziyanov

Abstract

Tyrosine site-specific recombinases (YRs) are widely distributed among prokaryotes and their viruses, and were thought to be confined to the budding yeast lineage among eukaryotes. However, YR-harboring retrotransposons (the DIRS and PAT families) and DNA transposons (Cryptons) have been identified in a variety of eukaryotes. The YRs utilize a common chemical mechanism, analogous to that of type IB topoisomerases, to bring about a plethora of genetic rearrangements with important physiological consequences in their respective biological contexts. A subset of the tyrosine recombinases has provided model systems for analyzing the chemical mechanisms and conformational features of the recombination reaction using chemical, biochemical, topological, structural, and single molecule-biophysical approaches. YRs with simple reaction requirements have been utilized to bring about programmed DNA rearrangements for addressing fundamental questions in developmental biology. They have also been employed to trace the topological features of DNA within high-order DNA interactions established by protein machines. The directed evolution of altered specificity YRs, combined with their spatially and temporally regulated expression, heralds their ...Continue Reading

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Citations

May 11, 2016·Chemical Reviews·Gretchen MeinkeFrank Buchholz
Feb 19, 2020·Molecular Biology and Evolution·Catherine BadelJacques Oberto
May 27, 2017·Nucleic Acids Research·Kushol GuptaGregory D Van Duyne
Oct 17, 2020·Scientific Reports·Eugenia VoziyanovaYuri Voziyanov
Feb 2, 2021·FEMS Microbiology Reviews·Catherine BadelJacques Oberto
Sep 26, 2021·Current Biology : CB·Aisling BradyJosé R Penadés

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