An Ovine Model for Studying the Pathophysiology of Septic Acute Kidney Injury

Methods in Molecular Biology
Yugeesh R LankadevaClive N May

Abstract

The development of acute kidney injury (AKI) is both a significant and independent prognostic factor of mortality in patients with sepsis, but its pathophysiology remains unclear. Herein, we describe an ovine model of sepsis evoked by the administration of live Escherichia coli in which there is hypotension, peripheral vasodilatation with a large increase in cardiac output; a similar hyperdynamic state to that commonly reported in humans. Interestingly, in this sheep model of sepsis, despite an increase in global kidney blood flow, there is a progressive reduction in renal function. Although renal hyperperfusion develops, renal tissue hypoxia due to redistribution of intrarenal blood flow may contribute to the pathogenesis of septic AKI. We have, therefore, developed a novel methodology to chronically implant combination probes to monitor intrarenal tissue perfusion and oxygen tension during the development of septic AKI in conscious sheep with hyperdynamic sepsis.

Citations

Oct 18, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yugeesh R LankadevaClive N May
May 30, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Naoya IguchiClive N May
Dec 12, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Roger G EvansYugeesh R Lankadeva
Jun 27, 2018·BMC Veterinary Research·T MartinelloM Patruno
Sep 16, 2020·Scientific Reports·Yugeesh R LankadevaRobin M McAllen
Jan 19, 2020·Research in Veterinary Science·I IacopettiM Patruno
Oct 9, 2021·Clinical Science·Thiele Osvaldt RosalesJamil Assreuy

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