An Oxidative Pathway for Microbial Utilization of Methylphosphonic Acid as a Phosphate Source.

ACS Chemical Biology
Simanga R GamaDavid L Zechel

Abstract

Methylphosphonic acid is synthesized by marine bacteria and is a prominent component of dissolved organic phosphorus. Consequently, methylphosphonic acid also serves as a source of inorganic phosphate (Pi) for marine bacteria that are starved of this nutrient. Conversion of methylphosphonic acid into Pi is currently only known to occur through the carbon-phosphorus lyase pathway, yielding methane as a byproduct. In this work, we describe an oxidative pathway for the catabolism of methylphosphonic acid in Gimesia maris DSM8797. G. maris can use methylphosphonic acid as Pi sources despite lacking a phn operon encoding a carbon-phosphorus lyase pathway. Instead, the genome contains a locus encoding homologues of the non-heme Fe(II) dependent oxygenases HF130PhnY* and HF130PhnZ, which were previously shown to convert 2-aminoethylphosphonic acid into glycine and Pi. GmPhnY* and GmPhnZ1 were produced in E. coli and purified for characterization in vitro. The substrate specificities of the enzymes were evaluated with a panel of synthetic phosphonates. Via 31P NMR spectroscopy, it is demonstrated that the GmPhnY* converts methylphosphonic acid to hydroxymethylphosphonic acid, which in turn is oxidized by GmPhnZ1 to produce formic acid ...Continue Reading

References

Nov 15, 1979·Analytical Biochemistry·P A LanzettaO A Candia
Jul 14, 2000·Nature·J J HudsonD W Schindler
Jan 29, 2009·Dalton Transactions : an International Journal of Inorganic Chemistry·J Martin BollingerCarsten Krebs
Jun 12, 2009·Nature·Robert M CicchilloWilliam W Metcalf
Apr 27, 2010·Chemistry & Biology·Benjamin T CircelloWilliam W Metcalf
Oct 19, 2011·Environmental Microbiology·Asunción MartínezSallie W Chisholm
Nov 18, 2011·Nature·Siddhesh S KamatFrank M Raushel
Feb 4, 2012·Frontiers in Microbiology·Juan F Villarreal-ChiuJohn W McGrath
May 9, 2012·Journal of the American Chemical Society·Fern R McSorleyDavid L Zechel
Apr 26, 2013·Nature·Siddhesh S KamatFrank M Raushel
Nov 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Bigna WörsdörferMaria-Eirini Pandelia
Dec 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Xiaomin YuWilliam W Metcalf
Dec 11, 2013·Frontiers in Microbiology·Asunción MartínezEdward F Delong
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Laura M van StaalduinenZongchao Jia
Feb 24, 2015·Journal of the American Chemical Society·Spencer C PeckWilfred A van der Donk
Aug 19, 2015·Nature·Paulina SewerynDitlev E Brodersen
Oct 28, 2016·Chemical Reviews·Geoff P Horsman, David L Zechel
Jan 17, 2017·Journal of the American Chemical Society·Spencer C PeckWilfred A van der Donk
Dec 1, 2017·Proceedings of the National Academy of Sciences of the United States of America·Israel A FigueroaJohn D Coates
Dec 9, 2017·Science·David A BornCatherine L Drennan
Mar 16, 2018·The Journal of Biological Chemistry·Michelle N GoettgeWilliam W Metcalf
Jun 19, 2018·Methods in Enzymology·Emily C UlrichDavid L Zechel

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Citations

Dec 4, 2019·Applied Biochemistry and Biotechnology·Natalia StosiekMagdalena Klimek-Ochab
Aug 11, 2021·Chembiochem : a European Journal of Chemical Biology·Simanga R GamaDavid L Zechel

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