An ozone-generating system and chamber for testing injury to cultured cells

Archives of Environmental Health
D G WenzelD L Morgan

Abstract

A system was constructed which alternately exposed cultured cells to specific concentrations of ozone in the gas phase and then to cell culture medium. It was designed to produce a constant mass transfer between the gas phase and the exposed cell phase. Monolayers of neonatal rat lung fibroblasts cultured in miniature glass dishes were used to test for ozone toxicity. The cells were alternately exposed to ozone (0.05, 0.15, 0.45, 1.35, and 4.05 ppm) for 30 sec and then to culture medium for 30 sec over a 1-hr period. Toxicity was measured as inhibition of cell growth 4 days after exposure to ozone. Growth was significantly inhibited at all concentrations with a 50% inhibitory concentration of 0.8 ppm. The system described was effective for quantifying ozone toxicity for cultured lung cells.

References

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Citations

Jul 1, 1985·Toxicology Letters·D L Morgan, D G Wenzel
Jun 8, 2001·Journal of Pharmacological and Toxicological Methods·P Laval-GillyM Henryon
Mar 1, 1984·Environmental Health Perspectives·M G Gabridge, M F Gladd
Jan 1, 1982·Drug and Chemical Toxicology·D G Wenzel, D L Morgan
Feb 26, 2013·Journal of Hazardous Materials·Faye F LiuJack C Ng
May 16, 2008·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Michaela Aufderheide
Jan 31, 2002·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·D RitterM Aufderheide
Aug 12, 2005·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Michaela Aufderheide
Mar 11, 2015·Chemosphere·Faye F LiuJack C Ng
Jan 1, 1985·Journal of Toxicology and Environmental Health·R Valentine

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