An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display

ACS Omega
Shigefumi KumachiNaoto Nemoto

Abstract

RNA-protein interactions have a central role in the living world. In this article, we examined whether primitive peptides (30 residues) consisting of four types of amino acid (Gly, Ala, Asp, and Val) could interact with tRNA as a model of primitive RNAs in the RNA world. By in vitro selection of binding peptides using the cDNA display method, a characteristic peptide was selected from a random peptide library and assayed by electrophoretic mobility shift and pull-down assays. Interestingly, the selected peptide bound to a single-stranded region including a loop structure of an RNA molecule with some sequence specificity.

References

Sep 7, 1995·Journal of Theoretical Biology·N Nemoto, Y Husimi
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·P SchimmelS Yokoyama
Jul 31, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Peter Tompa, Peter Csermely
Dec 3, 2004·Genes & Development·Haley Hieronymus, Pamela A Silver
Apr 14, 2005·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Kenji Ikehara
Jun 2, 2005·Protein Engineering, Design & Selection : PEDS·Nobuhide DoiHiroshi Yanagawa
Jan 22, 2008·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·H James CleavesJeffrey L Bada
Mar 18, 2008·FEBS Letters·Tina GlisovicGideon Dreyfuss
Oct 18, 2008·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Dimas A M ZaiaHenrique De Santana
Feb 18, 2010·Protein Science : a Publication of the Protein Society·Junko TanakaHiroshi Yanagawa
Jan 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Nathan J KuceraSandra L Wolin
Apr 20, 2011·Journal of Molecular Biology·Nitish MittalSarath Chandra Janga
Jul 20, 2011·ACS Combinatorial Science·Yuki MochizukiNaoto Nemoto
Jun 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Shingo UenoTakafumi Sakai
Apr 30, 2014·Chemical Reviews·Robin van der LeeM Madan Babu
Jun 27, 2015·Nature Communications·Sadeem AhmadRajan Sankaranarayanan
Sep 1, 2015·Journal of Biotechnology·Yuki MochizukiNaoto Nemoto

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Methods Mentioned

BETA
cDNA
PCR
cDNA display
Assay
Fluorescence
pull-down
electrophoresis

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