An RNAi Screen for Genes Required for Growth of Drosophila Wing Tissue

G3 : Genes - Genomes - Genetics
Michael D RotelliBrian R Calvi

Abstract

Cell division and tissue growth must be coordinated with development. Defects in these processes are the basis for a number of diseases, including developmental malformations and cancer. We have conducted an unbiased RNAi screen for genes that are required for growth in the Drosophila wing, using GAL4-inducible short hairpin RNA (shRNA) fly strains made by the Drosophila RNAi Screening Center. shRNA expression down the center of the larval wing disc using dpp-GAL4, and the central region of the adult wing was then scored for tissue growth and wing hair morphology. Out of 4,753 shRNA crosses that survived to adulthood, 18 had impaired wing growth. FlyBase and the new Alliance of Genome Resources knowledgebases were used to determine the known or predicted functions of these genes and the association of their human orthologs with disease. The function of eight of the genes identified has not been previously defined in Drosophila The genes identified included those with known or predicted functions in cell cycle, chromosome segregation, morphogenesis, metabolism, steroid processing, transcription, and translation. All but one of the genes are similar to those in humans, and many are associated with disease. Knockdown of lin-52, a ...Continue Reading

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Citations

Dec 23, 2020·Analytical Biochemistry·Thambi DoraiArthur J L Cooper

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Methods Mentioned

BETA
RNA-Seq
gene knockdown
GTPase

Software Mentioned

ImageJ
Excel
Fiji
DRSC Integrative Ortholog Prediction Tool ( DIOPT )
DIOPT
GraphPad Prism
modENCODE

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