An S-(hydroxymethyl)glutathione dehydrogenase is involved in conidiation and full virulence in the rice blast fungus Magnaporthe oryzae

PloS One
Zhen ZhangGuochang Sun

Abstract

Magnaporthe oryzae is a hemibiotrophic fungal pathogen that causes rice blast disease. A compatible interaction requires overcoming plant defense responses to initiate colonization during the early infection process. Nitric oxide (NO) plays important roles in defense responses during host-pathogen interactions. Microbes generally protect themselves against NO-induced damage by using enzymes. Here, we characterized an S-(hydroxymethyl)-glutathione dehydrogenase gene in M. oryzae, MoSFA1, the homologs of which are involved in NO metabolism by specifically catalyzing the reduction of S-nitrosoglutathione (GSNO) in yeasts and plants. As expected from the activities of S-(hydroxymethyl)glutathione dehydrogenase in formaldehyde detoxification and GSNO reduction, MoSFA1 deletion mutants were lethal in formaldehyde containing medium, sensitive to exogenous NO and exhibited a higher level of S-nitrosothiols (SNOs) than that of the wild type. Notably, the mutants showed severe reduction of conidiation and appressoria turgor pressure, as well as significantly attenuated the virulence on rice cultivar CO-39. However, the virulence of MoSFA1 deletion mutants on wounded rice leaf was not affected. An infection assay on barley leaf further re...Continue Reading

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Citations

Feb 18, 2016·Current Genetics·David CánovasJoseph Strauss
Mar 15, 2016·Frontiers in Plant Science·Magdalena Arasimowicz-Jelonek, Jolanta Floryszak-Wieczorek
Jan 15, 2021·International Journal of Molecular Sciences·Silvia ZaniniKarl-Heinz Kogel
Dec 29, 2020·Journal of Experimental Botany·Tereza JedelskáMarek Petřivalský
Dec 22, 2020·Frontiers in Plant Science·Shuanglong HuangW G Dilantha Fernando
Jul 20, 2017·Microbiology Spectrum·Alistair J P BrownJanet Quinn

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Methods Mentioned

BETA
PCR
light microscopy
optical microscopy

Software Mentioned

BlastP

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