An Ssn6-Tup1-dependent negative regulatory element controls sporulation-specific expression of DIT1 and DIT2 in Saccharomyces cerevisiae.

Molecular and Cellular Biology
Helena FriesenJ Segall

Abstract

Sporulation of the yeast Saccharomyces cerevisiae is a process of cellular differentiation that occurs in MATa/MAT alpha diploid cells in response to starvation. The sporulation-specific genes DIT1 and DIT2, which are required for spore wall formation, are activated midway through the sporulation program, with maximal transcript accumulation occurring at the time of prospore enclosure. In this study, we have identified a negative regulatory element, termed NREDIT, that is located between the start sites of transcription of these divergently transcribed genes. This element, which prevents expression of the DIT1 and DIT2 genes during vegetative growth, reduces expression of a CYC1-lacZ reporter gene more than 1,000-fold and acts in an orientation- and position-independent manner. We found that the ability of NREDIT to turn of expression of the reporter gene and the chromosomal DIT1 and DIT2 genes in vegetative cells requires the Ssn6-Tup1 repression complex. Interestingly, NREDIT-mediated repression of the reporter gene is maintained during sporulation. Derepression during sporulation requires complex interactions among several cis-acting elements. These are present on an approximately 350-bp DNA fragment extending from NREDIT to...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 1, 1992·Molecular and Cellular Biology·A K VershonA D Johnson
Feb 1, 1992·Molecular & General Genetics : MGG·E MatallanaJ E Pérez-Ortín
Mar 1, 1992·Microbiological Reviews·R S Zitomer, C V Lowry
Feb 21, 1992·Cell·C A KeleherA D Johnson
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R Elble, B K Tye
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L E BuckinghamR E Esposito
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L Guarente, E Hoar
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M J ClancyP T Magee
Jan 1, 1984·Molecular and Cellular Biology·A Percival-Smith, J Segall
Dec 1, 1995·Genes & Development·R L SmithA D Johnson
Apr 1, 1995·Current Opinion in Genetics & Development·S Y Roth
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·M A Treitel, M Carlson
Jun 1, 1995·Molecular and Cellular Biology·K S BowdishA P Mitchell
Jan 1, 1995·Trends in Genetics : TIG·H Ronne
Apr 1, 1994·Genes & Development·R StrichR E Esposito
Mar 1, 1994·Microbiological Reviews·A P Mitchell
May 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S J ElledgeT A Navas
Apr 1, 1993·Molecular and Cellular Biology·K S Bowdish, A P Mitchell
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·C M Steber, R E Esposito
Mar 1, 1996·Molecular and Cellular Biology·J OstlingH Ronne

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Citations

Dec 17, 2014·Cell Research·Li Yuan, Venkatesan Sundaresan
Oct 7, 2005·Microbiology·Chengshu WangRaymond J St Leger
Jan 11, 2000·Molecular and Cellular Biology·D DavisA P Mitchell
Sep 13, 2000·Molecular and Cellular Biology·A J KastaniotisR S Zitomer
Jan 18, 2006·Genetics·Martin LigrEric D Siggia
Feb 18, 1999·Molecular and Cellular Biology·S UfanoC R Vázquez de Aldana
May 10, 2000·Current Opinion in Cell Biology·A K Vershon, M Pierce
Mar 20, 1998·Genetics·P M Carrico, R S Zitomer
Dec 2, 1998·Genetics·J DeckertR S Zitomer
Oct 23, 1998·Science·S ChuI Herskowitz

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