An ultrasensitive electrochemical aptasensor for the determination of tumor exosomes based on click chemistry

Biosensors & Bioelectronics
Yu AnPingang He

Abstract

Exosomes, lipid bilayer membrane vesicles, can guide various pathological and physiological processes. However, reliable, convenient and sensitive methods for exosome determination for early cancer diagnosis are still technically challenging. Herein, an electrochemical aptasensor based on click chemistry and the DNA hybridization chain reaction (HCR) for signal amplification has been developed for the ultrasensitive detection of tumor exosomes. CD63 aptamer was first immobilized on a glassy carbon electrode for capturing exosomes, and 4-oxo-2-nonenal alkyne (alkynyl-4-ONE) molecules, functionalized lipid electrophiles, were conjugated to the exosomes via the reaction of amino and aldehyde groups. Azide-labeled DNA probe as an anchor was then connected to the exosomes by copper (I)-catalyzed click chemistry. Signal amplification was achieved by HCR, and the numerous linked horseradish peroxidase (HRP) molecules could catalyze the reaction of o-phenylenediamine (OPD) and H2O2. The concentration of exosomes could be quantified by monitoring the electrochemical reduction current of 2,3-diaminophenazine (DAP). Under the optimal conditions, this method allowed the sensitive detection of exosomes in the range of 1.12 × 102 to 1.12 × 1...Continue Reading

Citations

Aug 23, 2020·Materials·Jin-Ha ChoiJeong-Woo Choi
Apr 9, 2020·Frontiers in Chemistry·Rebeca Miranda-CastroNoemí de-Los-Santos-Álvarez
Aug 9, 2020·Critical Reviews in Analytical Chemistry·Xiaomeng YuJing Zhao
Oct 9, 2020·Chemistry, an Asian Journal·Zejun JiangJianping Li
Oct 24, 2020·Macromolecular Bioscience·Carmen Gutierrez-MillanClara I Colino
Jan 15, 2021·Diagnostics·Ragaa Abd-Ellatief, Maha Ragaa Abd-Ellatief
Jan 31, 2020·Biosensors & Bioelectronics·Wenhao DaiXueji Zhang
Feb 27, 2021·Analytical Chemistry·He YanYong Zeng
Mar 6, 2021·Chemical Reviews·Lingling WuWeihong Tan
Jun 3, 2021·International Journal of Molecular Sciences·Dhruvajyoti RoyJudith C Sporn
Aug 7, 2021·Nanoscale Advances·Meruyert ImanbekovaSebastian Wachsmann-Hogiu
Sep 10, 2020·Analytical Chemistry·Ayemeh Bagheri HashkavayiKi Soo Park
Sep 28, 2021·Frontiers of Medicine·Ying DengGenxi Li
Nov 9, 2021·Cancer Science·Tong MoXiaoyuan Chen

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