An ultrasensitive electrochemiluminescent immunosensor based on graphene oxide coupled graphite-like carbon nitride and multiwalled carbon nanotubes-gold for the detection of diclofenac

Biosensors & Bioelectronics
Liuyi HuJianguo Li

Abstract

In this study, a novel competition-type electrochemiluminescent (ECL) immunosensor for detecting diclofenac (DCF) was fabricated with graphene oxide coupled graphite-like carbon nitride (GO-g-C3N4) as signal probe for the first time. The ECL intensity of carboxylated g-C3N4 was significantly enhanced after being combined with graphene oxide (GO) which exhibited excellent charge-transport property. The sensing platform was constructed by multiwalled carbon nanotubes and gold nanoparticles (MWCNTs-AuNPs), which not only provided an effective matrix for immobilizing a large amount of coating antigen but also facilitated the electronic transmission rate to enhance the ECL intensity. Based on the synergistic effect of GO-g-C3N4 and MWCNTs-AuNPs composite, the proposed sensor showed high sensitivity, good stability, and wide linearity for the detection of DCF in the range of 0.005-1000ngmL-1 with a detection limit of 1.7pgmL-1. Furthermore, the developed immunoassay has been applied to real samples with satisfactory results. Therefore, this work provided a promising method for the detection of DCF and other small molecular compounds in the future.

References

Jul 31, 1998·Journal of Pharmaceutical and Biomedical Analysis·G Giagoudakis, S L Markantonis
Mar 4, 2000·Journal of Pharmaceutical and Biomedical Analysis·P C DamianiA C Olivieri
Sep 25, 2004·Ecotoxicology and Environmental Safety·Michael Cleuvers
Jul 30, 2008·Chemistry : a European Journal·Michael J BojdysArne Thomas
May 22, 2009·Analytical Chemistry·Susan D Richardson
Aug 1, 2009·Journal of the American Chemical Society·Xiufang ChenXinchen Wang
Nov 4, 2010·Journal of the American Chemical Society·Fangzheng SuXinchen Wang
Feb 8, 2011·Journal of the American Chemical Society·Yong WangMarkus Antonietti
Feb 22, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Qiao Liu, Junyan Zhang
Jun 1, 2015·Journal of Pharmaceutical Analysis·Bilal Yilmaz, Ulvihan Ciltas

❮ Previous
Next ❯

Citations

Sep 13, 2020·Environmental Science and Pollution Research International·Junia Teixeira MartinsPatricia Prediger
Aug 8, 2020·Analytical and Bioanalytical Chemistry·Wen-Zhi LinShao-Yi Hou
Nov 21, 2021·Analytical and Bioanalytical Chemistry·Alexander EckeRudolf J Schneider

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.